CARD HyperOva®

Great for IVF

Use CARD HyperOva® to obtain more ovulated oocytes.

PRODUCT OUTLINE

CARD HyperOva® (Japanese Patent No. 5927588) is an ultra-superovulation reagent that induces ovulation at higher efficiency when compared with the conventional superovulation induction method (PMSG-hCG method). Following the conventional method, we were only able to obtain approximaely 20 ova per mouse (C57BL/6J) on average; using CARD HyperOva® however, we can obtain as many as 3-4 times more ova.

EFFECTS

   Redction in Experimental Animal Use (ibution to 3R):

Using CARD HyperOva® enable us to reduce the number of female mice for ovum collection by 25 – 30 %.

   Optimization of Genetically Modified mouse Certion and Preservation:

More ova can be obtained from less female mice using CARD HyperOva® than following the conventional method, wich will facilitate in vitro fertilization and embrio transfare.

   Application to Valuable Genetically modified Mice:

Using CARD HyperOva® on your valuable genetically modified mice enables you to obtain a lot of ova from small number of females, thus enabling the large-scale preparation of embryos

SUPEROVULATION PROZEDURE:

1. Inject 0.1-0.2 mL CARD HyperOva® i.p. into a 26-30 days old female mouse (birthsdate= 0). Injections are usually performed during the light cycle, between 17:00 - 18:00.
2. At 48 hours after CARD HyperOva® recipients are injected i.p. with 7,5 IU human chorionic gonadotropin (hCG, not included).

Figure: (A) In conventional superovulation treatment, the number of oocytes increases by exogenous injection of eCG.
(B) By injecting anti-inhibin serum (AIS), endogenous inhibin secretion is neutralized and endogenous FSH secretion is maintained at a high level. This can induce a significantly larger number of developing follicles. (see reference 3.)

Referenzen:

1. Takeo T., Nakagata N. 2015. Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice. PLoS ONE 10(5): e0128330. doi:10.1371/journal.pone.0128330
2. Takeo T., Nakagata N. 2016. Immunotherapy using inhibin antiserum enhanced the efficacy of equine chorionic gonadotropin on superovulation in major inbred and outbred mice strains. Theriogenol. doi:10.1016/j.theriogenology.2016.04.076
3. Mochida, K., Hasegawa, A., & Ogura, A. 2017. Recent technical breakthroughs for ARTs in mice. Journal of Mammalian Ova Research, 34(1), 13-21.

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