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CARD HyperOva (Superovulation Reagent for mouse)

CARD HyperOva (Superovulation Reagent for mouse)

Item no.	KYD-010-EX
Manufacturer	Cosmobio
Amount	1 ml
Quantity options	600 ul 25 x 600 ul 5 x 600 ul 50 x 600 ul 1 ml 25 x 1 ml 5 x 1 ml 50 x 1 ml
Category	Assisted reproductive technologies Enhanced superovulation Reagents & Chemicals Molecules
Type	Bio-active substances
Specific against	other
Dry ice	Yes
Citations	Takeo T, Nakagata N. Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice. PLoS One. 2015 May 29;10(5).
ECLASS 10.1	32160490
ECLASS 11.0	32160490
UNSPSC	12000000
Available
Manufacturer - Category	IVF & Reproductive Engineering/Mouse
Shipping Temperature	Shipping dry ice
Storage Conditions	-20°C
Maker	KYD
Short Description	Save BIG (up to 11%) on multipacks and multipack bundles!\\CARD HyperOva® (Superovulation Reagent for mouse) is an ultra-superovulation reagent that is 3-4 times more efficient at inducing ovulation than the conventional PMSG-hCG method (80 ova per C57BL/6 mouse versus approximately 20). \\A mixture of equine chorionic gonadotropin and goat anti-inhibin antiserum, CARD HyperOva® is also effective with many other strains.
Description	CARD HyperOva^® (Superovulation Reagent for mouse) (Japanese Patent No. 5927588) is an ultra-superovulation reagent that is 3-4 times more efficient at inducing ovulation than the conventional PMSG-hCG method (80 ova per C57BL/6 mouse versus approximately 20). CARD HyperOva^® is also effective with many other strains. Even further gains in efficiency can be realized by using CARD MEDIUM^® during the IVF procedure. Why Use CARD HyperOva^®? Reduce Animal Use (contribute to the 3Rs of animal welfare: Replacement, Reduction, Refinement) Using CARD HyperOva^® can reduce the number of oocyte donor mice by a factor of 1/3-1/4. Optimize creation and preservation of genetically modified mice. More ova from fewer mice using CARD HyperOva^® facilitates in vitro fertilization and embryo transfer. More ova from fewer mice using CARD HyperOva^® enables the large-scale preparation of embryos. Get more done! For information on techniques for mouse spermatozoa cryopreservation, spermatozoa thawing, in vitro fertilization and obtaining pups through embryo transfer, please refer to the CARD website: http://card.medic.kumamoto-u.ac.jp/card/english/sigen/index.html
Composition	1. anti-inhibin antibody 2. Purified equine villus chorionic gonadotropin (eCG)
Protocol	1. Take CARD HyperOva out from the freezer, and thaw it in incubator or water bath at 37C for 10 minutes. 2. Administer the CARD HyperOva to mouse within one hour after thawing. Equalize the CARD HyperOVA by inverting the tube before aspirating it to syringe. 3. You may have some clumps floating* in the solution which you cannot aspirate by the injection needle. In such a case, avoid the clumps and aspirate only other solution. 4. Female mice (C57BL/6 strain, 4 weeks of age) are intraperitoneally administered CARD HyperOva (0.1 - 0.2mL/head; i.p). 5. At 48h after the administration of HyperOva, 7.5IU hCG(i.p.)** are administered to the mice. 6. At 17h after hCG injection, mice are sacrificed by cervical dislocation, and their oviducts are collected and transferred to a fertilization dish.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.