Recombinant Human sCD40 Ligand (Carrier-free)

CD40 Ligand (CD40L, CD154, TRAP, TNFSF5, gp39) belongs to the TNF superfamily and in its soluble form, displays some cytokine-like properties. CD40L binds to its receptor, CD40, to mediate T cell-dependent B cell responses and contribute to monocyte activation and dendritic cell maturation. Membrane-bound CD40L is expressed mainly on activated T cells, a small population of CD8+ T cells, and has been demonstrated on mast cells, basophils, eosinophils, B cells, NK cells and monocytes/macrophages. Activated platelets express CD40L on their surface and constitute the major source of soluble CD40L (sCD40L). sCD40L is a shorter protein than the membrane-bound version and retains biologic activity and ability to form trimers. CD40L expression can be upregulated using mitogens like PMA or PHA, through anti-CD3 activation, or by thrombin in platelets.

MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

Assay #1: Determined by the stimulation of IL-12 induction by human PBMCs.
Assay #2: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The expected ED₅₀ for this effect is 5-

Recombinant human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.

Angiogenesis/Cardiovascular; Apoptosis; Immune System; Inflammation; Proliferation; TNF Superfamily; Allergy; Transplantation

See Certificate of Analysis (COA) for lot specific reconstitution information.