Anti-IL-Beta antibody

Polyclonal

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

P01584

1 mg/ml

3553

Synthesized peptide derived from human IL-1beta.

Rabbit polyclonal against IL-beta

Cytoplasm
Cytosol
Lysosome
Secreted
Extracellular Exosome
The Precursor Is Cytosolic
In Response To Inflammasome-Activating Signals
Such As Atp For Nlrp3 Inflammasome Or Bacterial Flagellin For Nlrc4 Inflammasome
Cleaved And Secreted
Il1b Lacks Any Known Signal Sequence And The Pathway(S) Of Its Secretion Is(Are) Not Yet Fully Understood
On The Basis Of Experimental Results
Several Unconventional Secretion Mechanisms Have Been Proposed
1
Secretion Via Secretory Lysosomes: A Fraction Of Casp1 And Il1b Precursor May Be Incorporated
By A Yet Undefined Mechanism
Into Secretory Lysosomes That Undergo Ca(2+)-Dependent Exocytosis With Release Of Mature Il1b
2
Secretory Autophagy: Il1b-Containing Autophagosomes May Fuse With Endosomes Or Multivesicular Bodies (Mvbs) And Then Merge With The Plasma Membrane Releasing Soluble Il1b Or Il1b-Containing Exosomes
However
Autophagy Impacts Il1b Production At Several Levels And Its Role In Secretion Is Still Controversial
3
Secretion Via Exosomes: Atp-Activation Of P2rx7 Leads To The Formation Of Mvbs Containing Exosomes With Entrapped Il1b
Casp1 And Other Inflammasome Components
These Mvbs Undergo Exocytosis With The Release Of Exosomes
The Release Of Soluble Il1b Occurs After The Lysis Of Exosome Membranes (By Similarity)
4
Secretion By Microvesicle Shedding: Activation Of The Atp Receptor P2rx7 May Induce An Immediate Shedding Of Membrane-Derived Microvesicles Containing Il1b And Possibly Inflammasome Components
The Cytokine Is Then Released In The Extracellular Compartment After Microvesicle Lysis
5
Release By Translocation Through Permeabilized Plasma Membrane
This May Occur In Cells Undergoing Pyroptosis Due To Sustained Activation Of The Inflammasome (By Similarity)
These Mechanisms May Not Be Not Mutually Exclusive

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000

Interleukin-1 Beta
Il-1 Beta
Catabolin

IL1B_HUMAN