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Src Protein, Mouse, Recombinant (His & GST Tag)

Src Protein, Mouse, Recombinant (His & GST Tag)

Manufacturer	Sino Biological
Category	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Adenocarcinoma
Type	Proteins
Specific against	Mouse
Format	Baculovirus-Insect cells
Amount	50ug
Host	Baculovirus-Infected Insect Cells
Item no.	50311-M20B-50
eClass 6.1	34160400
eClass 9.0	42020190
Available
Alias	AW259666, pp60c-src
Molecule	SRC
Synonym	AW259666, pp60c-src
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Protein Construction	A DNA sequence encoding the mouse SRC (NP_001020566.111) (Met 1-Leu 535) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Purity	> 95 % as determined by SDS-PAGE
Bio-Activity	1. The specific activity was determined to be 166 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate. 2. Measured by its binding ability in a functional ELISA. Immobilized recombinant Mouse SRC at 2 ug/ml (100 ul/well) can bind biotinylated human PTPRA (aa 174-793) with a linear range of 0.032-0.8 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met
Molecule Mass	The recombinant mouse SRC/GST chimera consists of 773 amino acids and has a calculated molecular mass of 87.7 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0
Accession	NP_001020566.111
Amino acid sequence	Met1-Leu535
TAG	N-GST & His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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