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Human LIF Protein (Fc Tag)

Human LIF Protein (Fc Tag)

Item no.	14890-H02H-20
Manufacturer	Sino Biological
Amount	20ug
Category	Oncology Metabolism Diabetes Glucose Homeostasis Peptides & Proteins Recombinant Proteins Obesity By Organ Heart & Blood Cancer
Type	Proteins
Format	Human Cell
Specific against	Human
Host	Human
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CDF, DIA, HILDA, Leukemia Inhibitory Factor, MLPLI
Similar products	LIF
Available
Molecule	LIF
Synonym	CDF, DIA, HILDA, Leukemia Inhibitory Factor, MLPLI
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the human LIF (P15018)(Met1-Phe202) was expressed with the Fc region of human IgG1 at the C-terminus.
Expressed Host	Human Cells
Purity	> 95 % as determined by SDS-PAGE
Bio-Activity	1. Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.6-3 ng/ml. 2. Measured by its ability to bind human LIFR-His (Cat:10628-H08H) in a functional ELISA.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Ser 23
Molecule Mass	The recombinant human LIF/Fc is a disulfide-linked homodimer. The reduced monomer comprises 421 amino acids and has a predicted molecular mass of 46.7 kDa. The apparent molecular mass of the protein is approximately 63 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Accession	P15018
Amino acid sequence	Met1-Phe202
TAG	C-human IgG1-Fc

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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