Manufacturer |
Sino Biological
|
Category |
|
Type |
Proteins |
Specific against |
Human |
Amount |
100ug |
Item no. |
10457-H08H-100 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Alias |
AXT, FAME5, TAG-1, TAX, TAX1 |
Molecule |
CNTN2 |
Synonym |
AXT, FAME5, TAG-1, TAX, TAX1 |
Storage |
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Protein Construction |
A DNA sequence encoding the human CNTN2 (NP_005067.1) precursor (Met 1-Asn 1012) was expressed with a polyhistidine tag at the C-terminus. |
Expressed Host |
Human Cells |
Purity |
> 95 % as determined by SDS-PAGE |
Bio-Activity |
Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells
. When 5 x 10E4 cells/well are added to CNTN2-coated plates (0.8 ug/ml and 100 ul/well), approximately 40%-60% will adhere specifically after 60 minutes at 37C. |
Endotoxin |
< 1.0 EU per ug of the protein as determined by the LAL method |
Predicted N Terminal |
Ser 31 |
Molecule Mass |
The secreted recombinant human CNTN2 is a monomeric protein after cleavage of the signal peptide. It consists of 993 amino acids and predictes a molecular mass of 109 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 140 kDa due to glycosylation. |
Formulation |
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5 |
Accession |
NP_005067.1 |
Amino acid sequence |
Met1-Asn1012 |
TAG |
C-His |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.