Item no. |
13044-H07BL-300 |
Manufacturer |
Sino Biological
|
Amount |
300ug |
Category |
|
Type |
Cell Lysate |
Format |
Baculovirus-Insect cells |
Specific against |
Human |
Host |
Baculovirus-Infected Insect Cells |
ECLASS 10.1 |
32160890 |
ECLASS 11.0 |
32160890 |
UNSPSC |
41105330 |
Alias |
13044-H07BL |
Similar products |
TERF1 |
Available |
|
Molecule |
TERF1 |
Product Description |
Baculovirus-Insect Cells transfected lysate in which Human TERF1 / TRF1 has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer. |
Expressed Host |
Baculovirus-Insect cells |
Preparation Method |
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis Buffer |
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality Control Testing |
12.5% SDS-PAGE Stained with Coomassie Blue |
Recommend Usage |
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200uL pure water and boiled for 2-5 min. 3. Store it at -80C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. Notes: The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Storage Buffer |
In modified RIPA Lysis Buffer |
Storage Instruction |
Store at -80C. Aliquot to avoid repeated freezing and thawing |
Stability |
Samples are stable for up to twelve months from date of receipt at -80C |
Application notes |
WB: Use at an assay dependent dilution. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.