Item no. |
SEK50591-15 |
Manufacturer |
Sino Biological
|
Amount |
15 Plates |
Quantity options |
15 Plates
5 Plates
|
Category |
|
Type |
ELISA Pair Set |
Applications |
ELISA |
Specific against |
Mouse (Murine, Mus musculus) |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Alias |
SEK50591 |
Similar products |
EPCAM |
Available |
|
Manufacturer - Category |
ELISA |
Molecule |
EpCAM |
Storage |
Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles. |
Conjugate |
HRP |
Capture Ab |
1.0 mg/mL of rabbit anti-mouse EPCAM monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 ug/mL in CBS before coating. |
Detection Ab |
0.5 mg/mL rabbit anti-mouse EPCAM monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 ug/mL in detection antibody dilution buffer before use. |
Standard |
Each vial contains 400 ng of recombinant mouse EPCAM. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended. |
Sensitivity |
The minimum detectable dose of Mouse EpCAM / TROP-1 / TACSTD1 was determined to be approximately 78 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. |
Principle of the test |
The Mouse EpCAM / TROP-1 / TACSTD1 ELISA Pair Set is for the quantitative determination of Mouse EpCAM / TROP-1 / TACSTD1. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for EpCAM / TROP-1 / TACSTD1 coated on a 96-well plate. Standards and samples are added to the wells, and any EpCAM / TROP-1 / TACSTD1 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-EpCAM / TROP-1 / TACSTD1 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of EpCAM / TROP-1 / TACSTD1 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.