Comparison

Mouse CLEC4N Matched ELISA Antibody Pair Set European Partner

Item no. SEK50267-5
Manufacturer Sino Biological
Amount 5 Plates
Quantity options 15 Plates 5 Plates
Category
Type ELISA Pair Set
Applications ELISA
Specific against Mouse (Murine, Mus musculus)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias SEK50267
Similar products CLEC6A
Available
Manufacturer - Category
ELISA
Molecule
CLEC6A
Storage
Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Conjugate
HRP
Capture Ab
2 mg/mL of rabbit anti-mouse CLEC4N monoclonal antibody. Dilute to a working concentration of 1.0 ug/mL in CBS before coating.
Detection Ab
0.5 mg/mL rabbit anti-mouse CLEC4N polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 ug/mL in detection antibody dilution buffer before use.
Standard
Each vial contains 75 ng of recombinant mouse CLEC4N. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
Sensitivity
The minimum detectable dose of Mouse CLEC4N / Nkcl was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Principle of the test
The Mouse CLEC4N / Nkcl ELISA Pair Set is for the quantitative determination of Mouse CLEC4N / Nkcl.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CLEC4N / Nkcl coated on a 96-well plate. Standards and samples are added to the wells, and any CLEC4N / Nkcl present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CLEC4N / Nkcl polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CLEC4N / Nkcl present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5 Plates
Available: In stock
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