Comparison

Human Immunodeficiency Virus type 1 (HIV-1) p24 / Capsid Protein p24 ELISA Pair Set European Partner

Item no. SEK11695-5
Manufacturer Sino Biological
Amount 5 Plates
Quantity options 15 Plates 5 Plates
Category
Type ELISA Pair Set
Applications ELISA
Specific against Human Immunodeficiency Virus (HIV)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias SEK11695
Similar products GAG-P24
Available
Manufacturer - Category
ELISA
Molecule
Gag-p24
Storage
Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Conjugate
HRP
Capture Ab
1 mg/mL of mouse anti-HIV-1 P24 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 ug/mL in PBS before coating.
Detection Ab
0.2 mg/mL mouse anti-HIV-1 P24 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-protector, pH 7.4). Dilute to working concentration of 0.1 ug/mL in detection antibody dilution buffer before use.
Standard
Each vial contains 26 ng of recombinant HIV-1 P24. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
Sensitivity
The minimum detectable dose of HIV-1 p24 was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Principle of the test
The HIV-1 p24 ELISA Pair Set is for the quantitative determination of human HIV-1 p24.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for human immunodeficiency virus type 1 p24 coated on a 96-well plate. Standards andsamples are added to the wells, and any HIV-1 p24 present binds to theimmobilized antibody. The wells are washed and a horseradish peroxidaseconjugated mouse anti-HIV-1 p24 monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof HIV-1 p24 present in the sample. To end the enzyme reaction, the stopsolution is added and absorbances of the microwell are read at 450 nm.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5 Plates
Available: In stock
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