Human Immunodeficiency Virus type 1 (HIV-1) p24 / Capsid Protein p24 ELISA Pair Set

ELISA

Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.

1 mg/mL of mouse anti-HIV-1 P24 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 ug/mL in PBS before coating.

Each vial contains 26 ng of recombinant HIV-1 P24. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.

The HIV-1 p24 ELISA Pair Set is for the quantitative determination of human HIV-1 p24.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for human immunodeficiency virus type 1 p24 coated on a 96-well plate. Standards andsamples are added to the wells, and any HIV-1 p24 present binds to theimmobilized antibody. The wells are washed and a horseradish peroxidaseconjugated mouse anti-HIV-1 p24 monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof HIV-1 p24 present in the sample. To end the enzyme reaction, the stopsolution is added and absorbances of the microwell are read at 450 nm.