Human Carbonic Anhydrase XIV/CA14 Matched ELISA Antibody Pair Set

ELISA

Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.

0.5 mg/mL of rabbit anti-CA14 monoclonal antibody, Dilute to a working concentration of 2 ug/mL in CBS before coating.

Each vial contains 235 ng of recombinant CA14. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 8 ng/mL is recommended.

The Human Carbonic Anhydrase XIV / CA14 ELISA Pair Set is for the quantitative determination of Human Carbonic Anhydrase XIV / CA14.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Carbonic Anhydrase XIV / CA14 coated on a 96-well plate. Standards and samples are added to the wells, and any Carbonic Anhydrase XIV / CA14 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Carbonic Anhydrase XIV / CA14 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Carbonic Anhydrase XIV / CA14 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.