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Human CD157 Matched ELISA Antibody Pair Set

Human CD157 Matched ELISA Antibody Pair Set

Item no.	SEK10060-5
Manufacturer	Sino Biological
Amount	5 Plates
Quantity options	15 Plates 5 Plates
Category	Antibodies ELISA Immunoassay Antibody Pairs
Type	ELISA Pair Set
Applications	ELISA
Specific against	Human
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SEK10060
Similar products	BST1
Available
Manufacturer - Category	ELISA
Molecule	BST1
Storage	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles. Streptavidin-HRP: Store at 4C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
Conjugate	Biotin
Capture Ab	0.5 mg/mL of mouse anti-BST1 monoclonal antibody. Dilute to a working concentration of 1 ug/mL in CBS before coating.
Detection Ab	0.3 mg/mL of biotinylated rabbit anti-BST1 polyclonal antibody. Dilute to a working concentration of 0.2 ug/mL in detection antibody dilution buffer before use.
Standard	Each vial contains 15 ng of recombinant BST1. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1 ng/mL is recommended.
Streptavidin-HRP	50 uL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.
Sensitivity	The minimum detectable dose of human BST-1 / CD157 was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Principle of the test	The human BST-1 / CD157 ELISA Pair Set is for the quantitative determination of human BST-1 / CD157. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for BST-1 / CD157 coated on a 96-well plate. Standards and samples are added to the wells, and any BST-1 / CD157 present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-BST-1 / CD157 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color in proportion to the amount of BST-1 / CD157 present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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