Comparison

GFP Antibody, Mouse MAb European Partner

Manufacturer Sino Biological
Category
Type Antibody Monoclonal
Specific against other
Clone 05
Isotype IgG1
Applications IF, IP, ICC, ELISA
Amount 100ug
Host Mouse
Item no. 13105-MM05-100
eClass 6.1 32160702
eClass 9.0 42030590
Available
Alias 13105-MM05
Antigen molecule name
GFP
Storage
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Immunogen
Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)
Purification
Protein A
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Aequorea victoria GFP (Catalog#13105-S07E; AAB65663; Ser 2-Lys 238). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Specificity
Aequorea victoria GFP
No cross-reactivity
No cross-reactivity in ELISA with
E.coli cell lysate
Buffer
0.2 um filtered solution in PBS with 5% trehalose
ELISA
This antibody can be used at 0.5-1 ug/mL with the appropriate secondary reagents to detect Aequorea victoria GFP. The detection limit for Aequorea victoria GFP is approximately 0.16 ng/well.
Recommended ELISA Concentration
ELISA: 0.5-1 ug/mL
Recommended ICC-IF Concentration
ICC/IF: 10-25 ug/mL
Recommended IP Concentration
IP: 1-4 ug/mg of lysate

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100ug
Available: In stock
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