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Anti-IL-33 Antibody, Mouse Monoclonal

Anti-IL-33 Antibody, Mouse Monoclonal

Item no.	10368-MM03-200
Manufacturer	Sino Biological
Amount	200uL
Category	Antibodies Cell Biology Cell Culture Cytokines Flow Cytometry ELISA Immunofluorescence Immunocytochemistry Primary Antibodies
Type	Antibody Monoclonal
Applications	FC, IF, ICC, ELISA
Clone	03
Specific against	Human
Host	Mouse
Isotype	IgG2b
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	sino
Available
Antigen molecule name	IL33
Storage	This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Immunogen	Recombinant Human IL-33 protein (Catalog#10368-HNAE)
Purification	Protein A
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-33 (rh IL-33; Catalog#10368-HNAE; NP_254274.1; Ser 112-Thr 270). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Specificity	Human IL-33
No cross-reactivity	No cross activity in ELISA with Human cell lysate (293 cell line)
Buffer	0.2 um filtered solution in PBS with 5% trehalose
ELISA	This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect Human IL33. The detection limit for Human IL33 is approximately 0.625 ng/well.
Recommended ELISA Concentration	ELISA: 0.5-1.0 ug/mL
Recommended ICC-IF Concentration	ICC/IF: 10-25 ug/mL
Recommended FCM Concentration	FCM: 0.5-2 ug/Test

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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