PCR Videos

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abm - Your Source of PCR, qPCR, and RT Reagents and a Free Apple Watch

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  abm - qPCR Products Overview

  As an industry-leading and global biotech company, abm offers a wide range of qPCR enzyme products. Over the years, scientists have engineered the highest quality enzymes and most advanced and sophisticated formulations for qPCR applications. In addition, all of abm's products have been well validated by researchers around the world, with an increasing number of citations available in scientific publications. Learn more about:

  • Major strength of qPCR Mastermix
  • Advantages of ABM’s qPCR products
  • MasterMix selection guide
  • Platic Consumables in qPCR

  Fitting products:

  ABM-MasterMix-R BrightGreen 2X qPCR MasterMix-ROX
  ABM-MasterMix-ER BrightGreen Express 2X qPCR MasterMix-ROX
  ABM-G471-R One-Step BrightGreen qRT-PCR-ROX
  MasterMix-P TaqProbe 2X qPCR MasterMix-ROX
  ABM-G929 qPCR Control Template Kit
  ABM-G917-R ExCellenCT One-Step BrightGreen qRT-PCR-ROX
  ABM-G918-P ExCellenCT One-Step TaqProbe qRT-PCR-ROX
  ABM-G911 Opti-Seal qPCR Semi-skirted plate (Standard)

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  abm - PCR Products Overview

  As an industry-leading and global biotech company, abm offers a wide range of qPCR enzyme products. Over the years, scientists have engineered the highest quality enzymes and most advanced and sophisticated formulations for qPCR applications. In addition, all of abm's products have been well validated by researchers around the world, with an increasing number of citations available in scientific publications. Learn more about:

  • Polymerase selection guide
  • Bestaq performance
  • Examples of different PCR products

  Fitting products:

  ABM-G013 2X PCR Taq MasterMix
  ABM-G464 2X PCR Bestaq™ MasterMix
  ABM-G906-dye 2X PCR HotStart MasterMix with dye
  ABM-G124 2X PCR Precision™ MasterMix
  ABM-G923 Plant Ex-Amp PCR Kit (with Kodaq MasterMix)
  ABM-G108-G ExCellenCT One-Step BrightGreen qRT-PCR-ROX
  ABM-G918-P Safe-Green

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  1) PCR (Polymerase Chain Reaction) Tutorial - An Introduction

  This PCR introduction will demonstrate that PCR is a fundamental technique used to amplify fragments of DNA, frequently using the Taq polymerase to facilitate the amplification during the thermal cycling process. There are many variations of the PCR process including RT PCR for reverse transcription from an RNA template, qPCR for quantitative real time PCR to quantify the amplification during the chain reaction, and many more techniques to optimize the process. In this PCR video animation, you will be given an overview of the polymerase chain reaction steps as well as the role Taq DNA Polymerase plays in the PCR process. You will learn about PCR applications and factors important in the design of your PCR experiment.

  • Introduction
  • The PCR Process
  • Taq DNA Polymerase
  • Important factors in PCR

  Fitting products:

  ABM-G078 Precision™ DNA Polymerase (PCR)
  ABM-G462 Bloodirect DNA Polymerase (PCR)
  ABM-CH032-dye EDTA, Disodium Salt, Dihydrate
  ABM-G124 2X PCR Precision™ MasterMix
  ABM-G465 Bloodirect 2X PCR MasterMix
  ABM-E039 Bst DNA Polymerase, Large Fragment

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  2) Polymerase Chain Reaction (PCR) - DNA Polymerase

  DNA polymerases are essential for the all forms of PCR including real time quantitative PCR (qPCR), RT PCR, qRTPCR and reverse transcription, multiplex PCR, touch-down PCR and more. Polymerases are the responsible enzyme for nucleotide assembly and extension thus, essential to the assembly of new DNA molecules. However, there are a number of additional PCR components that also need to be taken into consideration. These included the thermal cycle conditions, fidelity, processivity and buffer components for the polymerase chain reaction. These DNA polymerases are indispensable to cell divisions as they duplicate the genetic information that would be passed to the next generation of daughter cells. Throughout the years, scientists have utilized different variants of DNA polymerases for different applications and prove to be essential for modern molecular biology.

  • DNA polymerase structure
  • Primers
  • Fidelity and processivity
  • Extension range
  • Types of DNA polymerases

  Fitting products:

  ABM-G009 Taq DNA Polymerase (PCR)
  ABM-E039 Bst DNA Polymerase, Large Fragment
  ABM-E043 Bsu DNA Polymerase, Large Fragment
  ABM-G011 HotStart DNA Polymerase (PCR)
  ABM-G498 Kodaq DNA Polymerase
  ABM-G460 Long-Range DNA Polymerase (PCR)
  ABM-G065 PCR-Sure™ Kit

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  3) Polymerase Chain Reaction (PCR) - Quantitative PCR (qPCR)

  Real-Time PCR or quantitative PCR (qPCR) is a PCR-based technique that is able to simultaneously amplify and detect changes in the amplicon concentration. Real-time PCR collects data during PCR amplification by utilizing fluorescence signals emitted by either special probes or DNA binding dyes.
  Among the numerous applications counts the quantitative analysis of gene expression, validating DNA microarray results or quantifying viral, bacterial or fungal loads. Learn more about:

  • Instruments used in qPCR
  • SYBR Green Base Method
  • Taqman Probe Method
  • FRET quenching and non-FRET static quenching
  • SYBR Green Method vs. Taqman Probe Method

  Fitting products:

  ABM-MasterMix-ER BrightGreen Express 2X qPCR MasterMix-ROX
  ABM-MasterMix-P TaqProbe 2X qPCR MasterMix-ROX
  ABM-C131 TaqMan Probe Design Service
  ABM-C334 Probe Synthesis (FAM/TAMRA)
  ABM-G918-P ExCellenCT One-Step TaqProbe qRT-PCR-ROX

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  How to extract genomic DNA from Plants - Plant Ex-Amp PCR Kit

  ABM's Plant Ex-Amp PCR Kit offers a streamlined, "vortex-boil-vortex" genomic DNA extraction solution perfect for obtaining PCR-ready templates in 15 minutes. Effective for extraction of genomic DNA from even the most challenging pine-tree-like samples, our Plant Ex-Amp Kit simplifies prepwork for PCR and quantitative real time PCR. This video shows you an example of a complete PCR workflow - from direct-from-plant genomic DNA extraction to PCR amplification to gel visualization. Make the Plant Ex-Amp Kit the foundation for your plant genetics research, today! Learn more about:

  • DNA extraction
  • PCR
  • Gel visualization

  Fitting products:

  ABM-G923 Plant Ex-Amp PCR Kit (with Kodaq MasterMix)
  ABM-G934 Plant Ex-Amp Extraction Buffer Set
  ABM-G497 Kodaq 2X PCR MasterMix
  ABM-G497-dye Kodaq 2X PCR MasterMix with dye
  ABM-? 10x Plant PCR Enhancer