NGS Videos

Erfahren Sie mehr über NGS

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  1) Next Generation Sequencing (NGS) - An Introduction

  Next Generation Sequencing (NGS) is a powerful platform that has enabled the sequencing of thousands to millions of DNA molecules simultaneously. This powerful tool is revolutionizing fields such as personalized medicine, genetic diseases, and clinical diagnostics by offering a high throughput option with the capability to sequence multiple individuals at the same time. Learn more about

  • Sanger Sequencing
  • NGS overview
  • Pyrosequencing
  • Sequencing by synthesis and by ligation
  • Comparisons across NGS platforms

  Fitting Products:

  ABM-G947S DNA Library Prep Kit for Illumina Sequencing (Purification beads included)
  ABM-IW20150/a> Whole Genome Sequencing (5Gb, 2x75bp, PE)
  ABM-IE30501 Exome Sequencing (Human, 45Mb Exome, 40X Coverage at Raw data level, 30X Average On-target Coverage)
  ABM-IA20001 Custom 16S Amplicon Primers
  ABM-IW10001 Full-Length Plasmid Sequencing (100Mb, 2x75bp PE)
  ABM-IW20150/a> RecA Protein, E. coli
  ABM-G950 DNA Purification SPRI Magnetic Beads
  ABM-E016 Exonuclease III, E. coli
  ABM-E007 E. coli DNA Ligase

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  2) Next Generation Sequencing (NGS) - Sample Preparation

  Major concepts in NGS (Next generation sequencing) are discussed, such as the science behind it and how to work with sequencing data. In this video, the steps to prepare samples for NGS in the specifics of each sequencing application are outlined. In this installment, the steps of library preparation for Whole Genome Sequencing, Exome Sequencing, RNA Sequencing and Methyl Sequencing on the Illumina platform are shown in detail.

  • Whole genome Sequencing
  • Exome sequencing
  • RNA sequencing
  • Methylation sequencing

  Fitting products:

  ABM-G947S DNA Library Prep Kit for Illumina Sequencing (Purification beads included)
  ABM-IW20100/a> Whole Genome Sequencing (1Gb, 2x75bp, PE)
  ABM-IE30501 Exome Sequencing (Human, 45Mb Exome, 40X Coverage at Raw data level, 30X Average On-target Coverage)
  ABM-IR13008 Total RNA Sequencing (8 million reads, 1x75bp SE)
  ABM-IW19001 DNA Isolation for NGS
  ABM-IS10001/a> Data Storage (per additional 3 months)

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  3) Next Generation Sequencing (NGS) - Coverage & Sample Quality Control

  Next Generation Sequencing (NGS) is a useful tool in deterring the DNA sequences, information which is valuable in further understanding biological processes. Compared to other tools, NGS is flexible and it can be used in different applications. However, there are parameters which have to be considered when running a NGS experiment. Here, the quantitative and qualitative measures needed to ensure a good sample for sequencing are outlined. In this installment the importance of having sufficient coverage and the process of quality control are described. Learn more about:

  • Coverage
  • Sample quality control
  • Quantitative validation
  • Qualitative validation

  Fitting products:

  ABM-G947S DNA Library Prep Kit for Illumina Sequencing (Purification beads included)
  ABM-IW19001/a> DNA Isolation for NGS
  ABM-IH10200 HiSeq 4000 High Output (1 Lane, 2x150nt, 400M Reads) Lane Rental
  ABM-IR18001 Sample and Library QC
  ABM-MasterMix-P TaqProbe 2X qPCR MasterMix-ROX

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  4) Next Generation Sequencing (NGS) - Data Analysis

  Next Generation Sequencing (NGS) is a useful tool in deterring the DNA sequences, information which is valuable in further understanding biological processes. In this installment, some of the common formats of NGS raw data and software that can be used for downstream analysis are described. Learn more about advantages of different software for your specific purpose and get a short introduction in bioinformatic data analysis programs. Step by step you are introduced how to deal with valuable NGS data. Get to know more about:

  • Raw data output
  • Sequence Alignment
  • Variant Calling
  • Additional Software & Tools

  Fitting products:

  ABM-IE30503 Exome Sequencing (Human, 45Mb Exome, 100X Coverage at Raw data level, 75X Average On-target Coverage)
  ABM-IB00015/a> Exome-Seq Analysis for 40X Raw data level, 30X Average On-target Coverage
  ABM-IW19001 DNA Isolation for NGS
  ABM-IS10001 Data Storage (per additional 3 months)
  ABM-IE30503 Exome Sequencing (Human, 45Mb Exome, 100X Coverage at Raw data level, 75X Average On-target Coverage)

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  5) Next Generation Sequencing (NGS) - Applications of Whole Genome Sequencing

  Whole Genome Sequencing is a powerful technology that is rapidly gaining popularity as it becomes more and more affordable. With whole genome sequencing, you can assemble genomes de novo, compare the genome of your organism to a reference genome, explore the molecular evolution of a species or population, accurately track pathogen outbreaks, and more. This video illustrates some real examples of whole genome sequencing applications. Learn more about

  • WGS (Whole Genome Sequencing) overview
  • De novo Genome Assembly
  • Pathogen Outbreaks
  • Molecular Evolution

  Fitting products:

  ABM-G934 Plant Ex-Amp Extraction Buffer Set
  ABM-G947S/a> DNA Library Prep Kit for Illumina Sequencing (Purification beads included)
  ABM-IR18001 DSample and Library QC
  ABM-IW20100 Whole Genome Sequencing (1Gb, 2x75bp, PE)
  ABM-IB00013 de novo Assembly (<10Mb Genome, Minimum 100X coverage needed)
  ABM-IB00001 Reference Genome Import (<10Mb genome)
  ABM-IB00011 Standard WGS Analysis