Item no. |
RLT-100-440S |
Manufacturer |
ReliaTech
|
Amount |
2ug |
Category |
|
Type |
Cytokines and Growth Factors |
Format |
Lyophilized |
Specific against |
Human |
Host |
HEK293 |
ECLASS 10.1 |
42030690 |
ECLASS 11.0 |
42030690 |
UNSPSC |
12352202 |
Alias |
Interleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF |
Available |
|
NCBI Gene ID |
3592 |
Uniprot |
P29460 |
Biological Activity |
Determined by its ability to increase IFN-? production by anti-TCR mAb-stimulated PBMCs. |
Description |
IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-? production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-?-producing Th1 cells. Recombinant Human IL-12 p70, derived from HEK293 cells, is a heterodimeric glycoprotein consisting of disulfide-linked p35 and p40 subunits (503 total amino acid residues). The calculated molecular weight of Human IL-12 p70 is 57.2 kDa; however, due to glycosylation, it migrates at an apparent molecular weight of 65-70 kDa based on SDS-PAGE gel, under non-reducing conditions. |
Endotoxin Levels |
< 0.1 ng/µg of protein (<1EU/µg) |
Length [aa] |
503 |
Molecular Weight |
65-70 kDa |
mRNA RefSeq |
NM_002187.2 |
Protein RefSeq |
NP_002178.2 |
Protein Sequence |
p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV V |
Purity Confirmation |
> 98% by SDS-PAGE & HPLC analyses |
Synonyms |
Interleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF |
Uniprot ID |
P29460 |
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