Item no. |
RLT-300-130 |
Manufacturer |
ReliaTech
|
Amount |
5ug |
Category |
|
Type |
Cytokines and Growth Factors |
Format |
Lyophilized |
Specific against |
Human, Mouse, Rat, Porcine |
Host |
E.coli |
ECLASS 10.1 |
42030690 |
ECLASS 11.0 |
42030690 |
UNSPSC |
12352202 |
Alias |
FGF4, HST, KFGF, HST-1, HSTF1, K-FGF, HBGF-4 |
Available |
|
NCBI Gene ID |
2249 |
Uniprot |
P08620 |
Biological Activity |
The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts). |
Buffer |
PBS |
Description |
FGF-4 (fibroblast growth factor 4), also known as K-FGF (Kaposi’s sarcoma associated FGF), is a 25 kDa secreted, heparin binding member of the FGF family. The human FGF-4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C terminus. Mature human FGF-4 shares a high aa identity with mouse, rat, canine and bovine FGF-4, respectively. The expression of FGF-4 and its receptors, FGF-R1c, -R2c, -R3c and R4, is spatially and temporally regulated during embryonic development. Its expression in the mouse trophoblast inner cell mass promotes expression of FGF-R2, and is required for maintenance of the trophectoderm and primitive endoderm. FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in-vitro. FGF-4 is mitogenic for fibroblasts and endothelial cells in-vitro and has autocrine transforming potential. It is a potent angiogenesis promoter in-vivo and has been investigated as therapy for coronary artery disease. |
Length [aa] |
177 |
Molecular Weight |
19.7 kDa |
mRNA RefSeq |
NM_002007 |
N Terminal Sequence |
MAPTAPNGTL |
Protein RefSeq |
NP_001998.1 |
Protein Sequence |
APTAPNGTLEAELERRWESLVALSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQALPDGRIGGAHADTRDSLLELSPVERGVVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFLP RL |
Purity Confirmation |
> 95% by SDS-PAGE & visualized by silver stain |
Reconstitution |
We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or -20 °C for future use. |
Stability And Storage |
The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20 °C. Reconstituted FGF-4 should be stored in working aliquots at -20 °C. Avoid repeated freeze-thaw cycles. |
Synonyms |
FGF4; HST; KFGF; HST-1; HSTF1; K-FGF; HBGF-4 |
Uniprot ID |
P08620 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.