Novel Green DNA Staining Reagent

Novel Green DNA Staining Reagent provides a 2-step method to stain DNA bands in DNA electrophoresis. It has higher sensitivity under UV transillumination, and is less mutagenic compared with ethidium bromide. It is provided as a 20, 000X concentrated reagent in DMSO and is compatible with TAE (40 mM Tris-acetate, 1 mM EDTA, pH 8), TBE (89 mM Tris base, 89 mM boric acid, 1 mM EDTA, pH 8), and TE (20 mM Tris base, 1 mM EDTA, pH 8) buffers. When excited at 248 nm, the fluorescent emission maxima of the Novel Green reagent is centred at 524 nm (after binding to DNA). The 10 × 50 ml size contains 5 × 50 ml Vial A (TMB in DMSO) and 5 × 50 ml Vial B (Sodium perboratetetrahydrate).

Dimethyl sulfoxide (DMSO).

Can be stored at room-temperature for short term storage. For long-term storage, aliquot store at -20 °C.