WB / Antibody Stripping Buffer

WB Stripping Buffer is used for recycling deproteinized membranes in western blot (WB). After carrying out a WB, a further WB may be need to be carried out with proteins with stable expression levels like actin and tubulin for reference, or to test other proteins for comparison. This reagent removes primary and secondary antibodies from the membrane by dissolving the conugation between primary and secondary antibodies and antigens, allowing the same membrane to be reused to detect other proteins. Compared with the SDS-PAGE gel, this reagent helps to eliminate the loading error and makes the experiment more comparable and accurate. This reagent is non-toxic, is free of β-Mercaptoethanol, and can be used at room temperature. This reagent can be used for at least 5 WB detections on the same membrane, and the whole detection can be completed within 15-30 min. This product is efficient and and does not affect proteins.

This product is for research use only.