Item no. |
230-10053-100 |
Manufacturer |
Raybiotech
|
Amount |
100 ug |
Category |
|
Type |
Proteins |
Format |
Lyophilized |
Applications |
WB, IP, ELISA, Dot |
Specific against |
Human (Homo sapiens) |
Host |
Human |
Conjugate/Tag |
HIS |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Pro-epidermal growth factor, EGF |
Shipping condition |
Cool pack |
Available |
|
Manufacturer - Applications |
Western blotting control, antibody validation (i.e., hybridoma screening, antibody pair screening), ELISA, EIA, dot blotting, immunoprecipitation (IP), protein array, protein-protein interaction studies, post-translational modifications |
Manufacturer - Conjugate / Tag |
N-terminal 8x histidine tag |
Manufacturer - Host |
Human |
Format |
Lyophilized powder |
Gene Symbols |
EGF |
Storage/Stability |
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles. |
Formulation |
Lyophilized from a 0.2 um filtered solution in PBS containing mammalian cell protease inhibitor cocktail |
Expressed Region |
Asn971-Arg1023 |
Protein Name & Synonyms |
Pro-epidermal growth factor, EGF |
Shipping |
Ice packs. Including one vial of HEK293 cell lysate transfected with empty expression vector. |
Purification |
Unpurified overexpression cell lysate |
Concentration (lot specific) |
Determined by BCA protein assay kit (Thermo Scientific) |
Reconstitution |
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
Endotoxin Level |
Not determined |
Preservative |
None |
Molecular Weight (kDa) |
45114 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.