Comparison

Recombinant Human EGF Protein, HEK293 Lysate

Item no. 230-10053-100
Manufacturer Raybiotech
Amount 100 ug
Category
Type Proteins
Format Lyophilized
Applications WB, IP, ELISA, Dot
Specific against Human (Homo sapiens)
Host Human
Conjugate/Tag HIS
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Pro-epidermal growth factor, EGF
Shipping condition Cool pack
Available
Manufacturer - Applications
Western blotting control, antibody validation (i.e., hybridoma screening, antibody pair screening), ELISA, EIA, dot blotting, immunoprecipitation (IP), protein array, protein-protein interaction studies, post-translational modifications
Manufacturer - Conjugate / Tag
N-terminal 8x histidine tag
Manufacturer - Host
Human
Format
Lyophilized powder
Gene Symbols
EGF
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS containing mammalian cell protease inhibitor cocktail
Expressed Region
Asn971-Arg1023
Protein Name & Synonyms
Pro-epidermal growth factor, EGF
Shipping
Ice packs. Including one vial of HEK293 cell lysate transfected with empty expression vector.
Purification
Unpurified overexpression cell lysate
Concentration (lot specific)
Determined by BCA protein assay kit (Thermo Scientific)
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level
Not determined
Preservative
None
Molecular Weight (kDa)
45114

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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