Item no. |
ELR-IL10-1 |
Manufacturer |
Raybiotech
|
Amount |
1 x 96-Well Strip Microplate Kit |
Category |
|
Type |
Elisa-Kit |
Specific against |
Rat |
Host |
E.coli |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Similar products |
IL-10 |
Available |
|
Quantity options |
|
Short description |
RayBio Rat IL-10 ELISA Kit for serum, plasma, and cell culture supernatants |
Target Name and Synonyms |
IL-10 |
Solid Support |
96-well Microplate |
Design Principle |
Sandwich-based |
Result Output |
Quantitative |
Method of Detection |
Colorimetric |
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum |
Sensitivity |
10 pg/ml |
Detection Range |
10 pg/ml - 6000 pg/ml |
Recommended Dilution (serum & plasma) |
2-10 fold |
Specificity |
This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., rat CINC-2, CINC-3, CNTF, IL-1alpha, IL-1beta, IL-4, IL-6, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF) |
Product Features |
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
|
Kit Components |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
|
Other Materials Required |
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
|
Protocol Summary |
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
|
Storage |
May be stored for up to 6 months at 2C to 8C from the date of shipment. Standard (recombinant protein) should be stored at -20C or -80C (recommended at -80C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2C to 8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20C. |
Citation |
1. Gullaiya S, Nagar A, Dubey V, et al. Modulation of disease related immune events by demethoxycurcumin against autoimmune arthritis in rats. Biomed Aging Pathol. 2013 Jan, 3(1):7–13. 2. Liu A., Pu Y., Zheng Y., Cai H., Bin Y. Therapeutic efficacies of chitosan against Pneumocystis pneumonia of immunosuppressed rat. Parasite Immunology doi: 10.1111/pim.12117 [Epub Ahead of Print] 3. Ahmed M., et al. Pomegranate extract protects against cerebral ischemia/reperfusion injury and preserves brain DNA integrity in rats. Life Sciences.Available July 2014. http://dx.doi.org/10.1016/j.lfs.2014.06.023 4. Alhusban A., et al. Compound 21is pro-angiogenic in the brain and results in sustained recovery after ischemic stroke. Journal of Hypertension, October 2014. doi: 10.1097/HJH.0000000000000364 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.