Item no. |
ELO-IL17A-1 |
Manufacturer |
Raybiotech
|
Amount |
1 Plate Kit |
Quantity options |
1 Plate Kit
2 Plate Kit
5 Plate Kit
|
Category |
|
Type |
Elisa-Kit |
Specific against |
Sheep (Ovine, Ovis aries) |
Host |
Hamster - Armenian |
Sensitivity |
0.45 pg/ml |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Alias |
Interleukin-17A (IL-17) (IL-17A) |
Shipping condition |
Cool pack |
Available |
|
Specificity |
This ELISA kit shows no cross-reactivity with the following cytokines tested: Ovine CXCL9 (MIG), Decorin, IFN-gamma, IL-8 (CXCL8), IL-21, IP-10 (CXCL10), sFRP-3, TNF-alpha, VEGF-A. |
Species Detected |
Ovine (sheep) |
Compatible Sample Types |
Cell Culture Supernatants Plasma Serum |
Design Principle |
Sandwich-based |
Method of Detection |
Colorimetric |
Quantitative/Semi-Quantitative |
Quantitative |
Solid Support |
96-well Microplate |
Kit Components |
Pre- Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin- Conjugated H R P T M B One- Step Substrate |
Other Materials Required |
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for E L I S A data analysis |
Protocol Outline |
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at R T or O/ N at 4° C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at R T. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at R T. Add 100 µl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately. |
Protein Name & Synonyms |
Interleukin-17A (IL-17) (IL-17A) |
Specicifity |
This ELISA kit shows no cross-reactivity with the following cytokines tested: Ovine CXCL9 (MIG), Decorin, IFN-gamma, IL-8 (CXCL8), IL-21, IP-10 (CXCL10), sFRP-3, TNF-alpha, VEGF-A. |
Recommended Dilution (serum & plasma) |
5 fold |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.