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Custom Human EGF Speed-E ELISA Kit

Custom Human EGF Speed-E ELISA Kit

Item no.	ELHS-EGF-1
Manufacturer	Raybiotech
Amount	1 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Shipping condition	Cool pack
Available
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	EGF
Kit Components	Streptavidin- Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Capture Antibody H R P Conjugated Detection Antibody T M B One- Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 Âµl to 1 Âµl volumes Adjustable 1-25 Âµl pipettes for reagent preparation100 Âµl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for E L I S A data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 70 Âµl of the Antibody Cocktail to each well. Incubate 10 minutes at R T. Add 50 Âµl of sample or standard to each well. Incubate 2 h at R T. Wash Wells Add 100 Âµl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 Âµl of Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability	May be stored for up to 6 months at 2Â° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2Â° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Protein Name & Synonyms	Pro-epidermal growth factor (EGF) [Cleaved into: Epidermal growth factor (Urogastrone)]

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

ELHS-EGF-1.pdf

ELHS-EGF-1-datasheet.pdf

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Amount: 1 Plate Kit

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Delivery expected until 1/24/2025

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