« Back
Home /
Human Ki-67/MKI67 ELISA Kit

Human Ki-67/MKI67 ELISA Kit

Item no.	ELH-MKI67-1
Manufacturer	Raybiotech
Amount	1 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Neural Development By Cell Type Blastoma Carcinoma Hematology Lymphoma Cell Biology Cell Status ELISA Proliferation Viability ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Bladder Cancer Breast Cancer Testicular & Prostate Cancer Cytoskeleton Markers
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	0.045 ng/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Shipping condition	Cool pack
Available
Specificity	This ELISA antibody pair detects Human MKI67. Other species not determined.
Compatible Sample Types	Cell Culture Supernatants Plasma Serum
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Kit Components	Pre- Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin- Conjugated H R P T M B One- Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 Âµl to 1 Âµl volumes Adjustable 1-25 Âµl pipettes for reagent preparation100 Âµl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for E L I S A data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 Âµl of standard or sample to each well. Incubate 2.5 h at R T or O/ N at 4Â° C. Add 100 Âµl of prepared biotin antibody to each well. Incubate 1 h at R T. Add 100 Âµl of prepared Streptavidin solution to each well. Incubate 45 min at R T. Add 100 Âµl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 Âµl of Stop Solution to each well. Read at 450 nm immediately.
Protein Name & Synonyms	Proliferation marker protein Ki-67 (Antigen identified by monoclonal antibody Ki-67) (Antigen KI-67) (Antigen Ki67)
Specicifity	This ELISA antibody pair detects Human MKI67. Other species not determined.
Recommended Dilution (serum & plasma)	3 fold
Gene Names	MKI67

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

ELH-MKI67-1.pdf

ELH-MKI67-1-datasheet.pdf

ELH-MKI67-1-safety-datasheet.pdf