Comparison

Human Oncostatin M R beta ELISA Kit

Item no. ELH-OSMRb-2
Manufacturer Raybiotech
Amount 2 Plate Kit
Quantity options 1 Plate Kit 2 Plate Kit 5 Plate Kit
Category
Type Elisa-Kit
Specific against Human (Homo sapiens)
Host Hamster - Armenian
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Oncostatin-M-specific receptor subunit beta (Interleukin-31 receptor subunit beta) (IL-31 receptor subunit beta) (IL-31R subunit beta) (IL-31R-beta) (IL-31RB)
Shipping condition Cool pack
Available
Specificity This ELISA antibody pair recognizes human Oncostatin M R beta. Other species not yet determined.
Design Principle
Sandwich-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Quantitative
Solid Support
96-well Microplate
Gene Symbols
OSMROSMRB
Kit Components
Pre-
Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-
Conjugated H
R
P
T
M
B One-
Step Substrate
Other Materials Required
Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for E
L
I
S
A data analysis
Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at R
T or O/
N at 4°
C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at R
T.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at R
T.
Add 100 µl of T
M
B One-
Step Substrate Reagent to each well.
Incubate 30 min at R
T.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
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Biotech's Speed
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L
I
S
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Protein Name & Synonyms
Oncostatin-M-specific receptor subunit beta (Interleukin-31 receptor subunit beta) (IL-31 receptor subunit beta) (IL-31R subunit beta) (IL-31R-beta) (IL-31RB)
Specicifity
This ELISA antibody pair recognizes human Oncostatin M R beta. Other species not yet determined.
Additional Notes
Strip plates and additional reagents allow for use in multiple experimentsQuantitative protein detectionEstablishes normal rangeThe best products for confirmation of antibody array data
Note
This ELISA uses an antibody pair and standard that have been validated for multiplex array products, but not for the 96-well plate ELISA format. Thus, this ELISA requires a 4 - 7 week development phase, which RayBiotech begins after receiving an order for the kit. After development, the kit will have passed RayBiotech's ELISA quality control tests, and will thereafter have a typical 1 - 2 day lead time for orders placed.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 2 Plate Kit
Available: In stock
available

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