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Human IL-4 R alpha ELISA Kit

Human IL-4 R alpha ELISA Kit

Item no.	ELH-IL4Ra-2
Manufacturer	Raybiotech
Amount	2 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	0.04 ng/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Interleukin-4 receptor subunit alpha (IL-4 receptor subunit alpha) (IL-4R subunit alpha) (IL-4R-alpha) (IL-4RA) (CD antigen CD124) [Cleaved into: Soluble interleukin-4 receptor subunit alpha (Soluble IL-4 receptor subunit alpha) (Soluble IL-4R-alpha) (sIL4Ralpha/prot) (IL-4-binding protein) (IL4-BP)]
Shipping condition	Cool pack
Available
Specificity	This ELISA antibody pair detects human IL-4 R alpha. Other species not determined.
Compatible Sample Types	Cell Culture Supernatants Plasma Serum
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Kit Components	Pre- Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin- Conjugated H R P T M B One- Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 Âµl to 1 Âµl volumes Adjustable 1-25 Âµl pipettes for reagent preparation100 Âµl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for E L I S A data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 Âµl of standard or sample to each well. Incubate 2.5 h at R T or O/ N at 4Â° C. Add 100 Âµl of prepared biotin antibody to each well. Incubate 1 h at R T. Add 100 Âµl of prepared Streptavidin solution to each well. Incubate 45 min at R T. Add 100 Âµl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 Âµl of Stop Solution to each well. Read at 450 nm immediately.
Specicifity	This ELISA antibody pair detects human IL-4 R alpha. Other species not determined.
Recommended Dilution (serum & plasma)	2 fold
Gene Names	582J2.1IL4RIL4RA
Antigen Name & Synonyms	Interleukin-4 receptor subunit alpha (IL-4 receptor subunit alpha) (IL-4R subunit alpha) (IL-4R-alpha) (IL-4RA) (CD antigen CD124) [Cleaved into: Soluble interleukin-4 receptor subunit alpha (Soluble IL-4 receptor subunit alpha) (Soluble IL-4R-alpha) (sIL4Ralpha/prot) (IL-4-binding protein) (IL4-BP)]

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

ELH-IL4Ra-2.pdf

ELH-IL4Ra-2-datasheet.pdf

ELH-IL4Ra-2-safety-datasheet.pdf