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Human TGF-beta 1 ELISA

Human TGF-beta 1 ELISA

Item no.	ELH-TGFb1-5
Manufacturer	Raybiotech
Amount	5 Plate Kit
Quantity options	1 x 96-Well Strip Microplate Kit 2 Plate Kit 5 Plate Kit
Category	Oncology Neuroscience Neuroinflammation By Cell Type Carcinoma Cell Biology Cell Culture Cytokines Mesothelioma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Colorectal Cancer
Type	Elisa-Kit
Specific against	Human
Host	Hamster - Armenian
Sensitivity	80 pg/ml
Citations	1. Zou W, Zou Y, Zhao Z, et al. Nicotine-induced epithelial-mesenchymal transition via Wnt/beta-catenin signaling in human airway epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol. 2013 Feb 15,304(4):L199–209.<br />2. Kanlaya R, Sintiprungrat K, Thongboonkerd V. Secreted Products of Macrophages Exposed to Calcium Oxalate Crystals Induce Epithelial Mesenchymal Transition of Renal Tubular Cells via RhoA-Dependent TGF-beta1 Pathway. Cell Biochem. Biophys. 2013 May 23, [Epub ahead of print] doi:10.1007/s12013-013-9639-z
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	TGF beta 1
Available
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70)
Quantity options
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Urine
Design Principle	Sandwich-based
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Method of Detection	Colorimetric
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Protocol Summary	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Recommended Dilution (serum & plasma)	3 fold after treatment (see manual)
Result Output	Quantitative
Short description	RayBio Human Transforming growth factor-beta 1 ELISA Kit for serum, plasma, cell culture supernatants, and urine
Solid Support	96-well Microplate
Storage	May be stored for up to 6 months at 2C to 8C from the date of shipment. Standard (recombinant protein) should be stored at -20C or -80C (recommended at -80C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2C to 8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20C.
Target Name and Synonyms	TGF beta 1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.