Item no. |
ELH-IL1a-CL-2 |
Manufacturer |
Raybiotech
|
Amount |
2 Plate Kit |
Quantity options |
1 x 96-Well Strip Microplate Kit
2 Plate Kit
5 Plate Kit
|
Category |
|
Type |
Elisa-Kit |
Specific against |
Human |
Host |
Hamster - Armenian |
Sensitivity |
0.5 pg/ml |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Similar products |
IL-1 alpha, IL-1 F1 |
Available |
|
Specificity |
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN- |
Quantity options |
|
Compatible Sample Types |
Tissue Lysates, Cell Lysates |
Design Principle |
Sandwich-based |
Kit Components |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
|
Method of Detection |
Colorimetric |
Other Materials Required |
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
|
Product Features |
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
|
Protocol Summary |
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
|
Result Output |
Quantitative |
Short description |
RayBio Human IL-1 alpha ELISA Kit for cell and tissue lysates |
Solid Support |
96-well Microplate |
Storage |
May be stored for up to 6 months at 2C to 8C from the date of shipment. Standard (recombinant protein) should be stored at -20C or -80C (recommended at -80C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2C to 8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20C. |
Target Name and Synonyms |
IL-1 alpha, IL-1 F1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.