Human beta-NGF ELISA

RayBio Human beta-NGF ELISA Kit for serum, plasma, cell culture supernatants, and urine

96-well Microplate

Quantitative

Cell Culture Supernatants, Plasma, Serum, Urine

14 pg/ml - 5000 pg/ml

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 ul of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4C.
4. Add 100 ul of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 ul of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 ul of Stop Solution to each well.
11. Read at 450 nm immediately.

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2. Lambiase, Alessandro, Mauro P. Ferrari, and Flavio Mantelli. "Safety and Pharmacokinetics of Escalating Doses of Human Recombinant Nerve Growth Factor Eye Drops in a Double-Masked, Randomized Clinical Trial." BioDrugs. Springer Link, Dec. 2013. Web.
3. Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111.