« Back
Home /
Human BDNF ELISA

Human BDNF ELISA

Item no.	ELH-BDNF-5
Manufacturer	Raybiotech
Amount	5 Plate Kit
Quantity options	1 x 96-Well Strip Microplate Kit 2 Plate Kit 5 Plate Kit
Category	Oncology ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Bladder Cancer Renal Cancer
Type	Elisa-Kit
Specific against	Human
Host	Hamster - Armenian
Sensitivity	80 pg/ml
Citations	1. Browne AS., et al. Proteomic identification of neurotrophins in the eutopic endometrium of women with endometriosis. Fertil Steril. 2012 Sep,98(3):713-9. doi: 10.1016/j.fertnstert.2012.05.027. <br />2. Uguz F, Sonmez EO, Sahingoz M, et al. Maternal generalized anxiety disorder during pregnancy and fetal brain development: A comparative study on cord blood brain-derived neurotrophic factor levels. J Psychosomatic Res. 2013 Aug 5, [Epub ahead of print] doi:10.1016/j.jpsychores.2013.07.002.<br />3. Oral E, Ozcan H, Kirkan TS, et al. Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder. Eur Arch Psychiatry Clin Neurosci. 2013 Mar 2, [Epub ahead of print] doi:10.1007/s00406-013-0398-z.<br />4. Browne A., Yu J., Huang R., et al. Proteomic identification of neurotrophins in the eutopic endometrium of women with endometriosis Fertility and Sterility Vol 98, Issue 3, September 2012, Pages 713–719<br />5. Liu Y., et al. Associations Between ApoEepsilon4 Carrier Status and Serum BDNF Levels—New Insights into the Molecular Mechanism of ApoEepsilon4. Mol Neurobio. Online First June 2014. DOI 10.1007/s12035-014-8804-8 Actions in Alzheimer’s Disease<br />6. Sinkevicius K., et al. Neurotrophin receptor TrkB promotes lung adenocarcinoma metastasis. PNAS 2014 111 (28) 10299-10304, published ahead of print June 30, 2014, doi:10.1073/pnas.1404399111<br />7. Sahin S., et al. Effect of Methylphenidate Treatment on Appetite and Levels of Leptin, Ghrelin, Adiponectin, and Brain-Derived Neurotrophic Factor in Children and Adolescents with Attention Deficit and Hyperactivity Disorder. International Journal of Pyschiatry in Clinical Practice. Posted online on July 4, 2014. (doi:10.3109/13651501.2014.940054)<br />8. Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111. <br />9. Perez-Garcia JL., et al. Identification of TNF-a and MMP-9 as potential baseline predictive serum markers of sunitinib activity in patients with renal cell carcinoma using a human cytokine array. British Journal of Cancer (2009) 101, 1876 – 1883
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	BDNF
Available
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF
Compatible Sample Types	Cell Culture Supernatants Plasma Serum
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	BDNF
Kit Components	Pre- Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin- Conjugated H R P T M B One- Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for E L I S A data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at R T or O/ N at 4° C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at R T. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at R T. Add 100 µl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately. Need your results faster? Try Ray Biotech's Speed E L I S A platform for quantitative detection in just three hours.
Storage/Stability	The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Protein Name & Synonyms	BDNF
Recommended Dilution (serum & plasma)	5 - 500 fold
Additional Notes	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Spiking & Recovery Results	Sample Type Average % Recovery Range (%) Serum 94.57 83-103 Plasma 92.48 84-102 Cell culture media 93.35 83-102
Linearity Results	Serum Plasma Cell Culture Media 1:2 Average % of expectedRange (%) 9383-103 9284-103 9483-104 1:4 Average % of expectedRange (%) 9484-104 9383-102 9585-103

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.