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Human Aminopeptidase N/ANPEP ELISA

Human Aminopeptidase N/ANPEP ELISA

Item no.	ELH-ANPEP-1
Manufacturer	Raybiotech
Amount	1 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	Oncology Neuroscience Molecular Targets for Neuroscience By Cell Type Hematology Leukemia ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human
Host	Hamster - Armenian
Sensitivity	40 pg/ml
ECLASS 5.1	32160605
ECLASS 6.1	32160605
ECLASS 8.0	32160605
ECLASS 9.0	32160605
ECLASS 10.0.1	32160605
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Specificity	This ELISA antibody pair recognizes Human Aminopeptidase N.
Compatible Sample Types	Cell Culture Supernatants Plasma Serum
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	ANPEPAPNCD13PEPN
Kit Components	Pre- Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin- Conjugated H R P T M B One- Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for E L I S A data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at R T or O/ N at 4° C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at R T. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at R T. Add 100 µl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately. Need your results faster? Try Ray Biotech's Speed E L I S A platform for quantitative detection in just three hours.
Protein Name & Synonyms	Aminopeptidase N (AP-N) (hAPN) (EC 3.4.11.2) (Alanyl aminopeptidase) (Aminopeptidase M) (AP-M) (Microsomal aminopeptidase) (Myeloid plasma membrane glycoprotein CD13) (gp150) (CD antigen CD13)
Recommended Dilution (serum & plasma)	1, 000 fold
Additional Notes	Strip plates and additional reagents allow for use in multiple experimentsQuantitative protein detectionEstablishes normal rangeThe best products for confirmation of antibody array data

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.