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Human ZAG EIA

Human ZAG EIA

Item no.	EIA-ZAG-5
Manufacturer	Raybiotech
Amount	5 Plate Kit
Quantity options	1 x 96-Well Strip Microplate Kit 2 Plate Kit 5 Plate Kit
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human
Host	Hamster - Armenian
Sensitivity	5.4 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	ZAG
Available
Specificity	This kit detects ZAG 278aa. No other active isoforms have been reported.Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, NPY and APC.
Species Detected	Human Mouse Rat
Compatible Sample Types	Cell Culture Supernatants Serum
Design Principle	Competition-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	AZGP1ZAGZNGP1
Kit Components	Pre- Coated 96-well Strip Microplate Wash Buffer Standard Peptide Assay Diluent(s) Biotinylated Peptide H R P- Streptavidin T M B One- Step Substrate Stop Solution Assay Diagram Positive Control Sample Capture Antibody User Manual
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation100 ml and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Orbital shaker Aluminum foil Saran Wrap Absorbent paper Microplate reader capable of measuring absorbance at 450nm Sigma Plot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline	Prepare all reagents, samples and standards as instructed. Add 100 µl detection antibody to each well. Incubate 1.5 h at R T or O/ N at 4° C. Add 100 µl standard or sample to each well. Incubate 2.5 h at R T. Add 100 µl prepared streptavidin solution. Incubate 45 min at R T. Add 100 µl T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 µl Stop Solution to each well. Read plate at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Protein Name & Synonyms	Zinc-alpha-2-glycoprotein (Zn-alpha-2-GP) (Zn-alpha-2-glycoprotein)
Recommended Dilution (serum & plasma)	Human: 8X / Mouse: 2X / Rat: 10X

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.