« Back
Home /
Lectin Array 70

Lectin Array 70

Item no.	GA-Lectin-70-56
Manufacturer	Raybiotech
Amount	56 Sample Kit
Quantity options	14 Sample Kit 28 Sample Kit 56 Sample Kit
Category	Arrays Protein Arrays
Type	Array
Specific against	other
Host	Hamster - Armenian
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Shipping condition	Cool pack
Available
Manufacturer - Applications	Identify and profile the glycans in their samples Determine whether their biomarker of interest has glycan moieties Find specific glycan binding ligands in biological samples
Manufacturer - Category	Arrays\|Glycobiology Arrays
Shipping Temperature	Blue ice
Storage Conditions	Upon receipt, all components of the Raybiotech Lectin Array 70 kit should be stored at -20°C. Once thawed, the glass slide and Cy3 equivalent dye-conjugated Streptavidin should be kept at -20°C and all other components may be stored at 4°C. The entire kit should be used within 6 months of purchase.
Number of Targets Detected	70
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates
Design Principle	Label-basedSandwich-based
Method of Detection	Fluorescence Laser Scanner
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Glass Slide
Kit Components	Dialysis Vials Labeling Reagent Labeling Buffer Stop Solution Lectin Array Glass Slide Assembly Sample Diluent20 X Wash Buffer I20 X Wash Buffer IICy3 equivalent dye-conjugated Streptavidin Slide Washer/Dryer Adhesive device sealer Floating Dialysis Rack Manual
Other Materials Required	Detection antibodies of interest ( For sandwich-based method only) Orbital shaker Laser scanner for fluorescence detection Aluminum foil1.5ml Polypropylene microcentrifuge tubesKCl, Na Cl, K H2PO4 and Na2HP O4 ( For label-based method only) Plastic or glass containers, beaker, stir plate and stir bar Pipettors, pipette tips, dd H2 O and other common lab consumables
Protocol Outline	Dry the glass slide Block array surfaces Incubate samples (samples need to be biotinylated for the label-based approach) For the the sandwich-based principle, incubate with a detection antibody cocktail For the label-based principle, incubate the labeled-streptavidin. Incubate with Cy3 Equivalent Dye-Streptavidin Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
UNSPSC Code	41116133
Application Notes	Quantitative analysis of lectin-glycoprotein interactions. Example: a concentration series of glycoproteins detected with the lectin array could reveal concentration dependent effects of lectin-glycan binding.Determine the profile of bacterial cell-surface glycans. Example: Cell lysate from bacteria can be Biotinylated and hybridized to the lectin array. Analysis of the binding pattern and correlation with the known carbohydrate-binding specificities of the lectins can determine the glycans on the cell membrane.
Product Feature	High sensitivity and specificity Low sample volume (10-100 µl per array) Large dynamic range of detection Compatible with most sample types Test 14 samples on each slide Suitable for high-throughput assays
Application 1 - Detection of Glycans on a Purified Protein	See Image/s In this application, the RayBio Lectin Array 70 was used to detect specific glycosylations of purified Horseradish Peroxidase (HRP). Lectins BANLEC, BC2L-A, CALSEPA, GNA, HHA, NPA, PA-IIL, and PALa showed strong signals after incubation with 3.3 ug/mL Biotin-HRP followed by detection with streptavidin-fluorescence-dye (Figures A, B and C). The fluorescence signals from BANLEC, BC2L-A, CALSEPA, GNA, HHA, NPA, PA-IIL, and PALa were blocked in a concentration-dependent manner by HRP itself (Figures A and C), indicating that the signals were generated by lectin-HRP binding. These eight lectins are known to exhibit specific binding to mannose, which indicates that HRP contains mannose. After adding increasing amounts of mannose, the signal from BANLEC, BC2L-A, CALSEPA, GNA, HHA, NPA, PA-IIL, and PALa were reduced (Figures A and B).The reduction in signals from increasing concentrations of mannose confirms that HRP protein contains mannose in its glycocalyx. Additionally, the two lectins AAL and RS-FUC (fucose binding specificity) also showed strong interaction with HRP, which indicates the fucosylation of HRP. Overall, the results of the Lectin Array 70 were consistent with published literature regarding HRP glycosylation.
Application 2 - Profiling of a Serum Sample	See Image/s Using the lectin array, we can discover the different glycoprotein profiles of the serum samples, cell lysates, or purified glycoprotein. The images above show the profiles of the glycans from different types of samples including human serum, recombinant glycoproteins human HE4, AFP, mouse TTF, purified human IgG, and bacterial cell lysates OF DH-5Î±, DE3 detected by Biotin labeling and Fluorescence dye-streptavidin.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.