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Bradykinin ELISA Kit

Bradykinin ELISA Kit

Item no.	EIA-BRAK-1
Manufacturer	Raybiotech
Amount	1 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Category	ELISA & Immunoassays Immunoassay EIA Kits
Type	EIA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Hamster - Armenian
Sensitivity	1.4 ng/ml
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Shipping condition	Cool pack
Available
Specificity	This EIA kit is designed to detect human, mouse, and rat Dynorphin
Compatible Sample Types	Cell Culture Supernatants Plasma Serum
Design Principle	Competition-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	BDKKNGKNG1
Kit Components	Pre- Coated 96-well Strip Microplate Wash Buffer Standard Peptide Assay Diluent(s) Biotinylated Peptide H R P- Streptavidin T M B One- Step Substrate Stop Solution Assay Diagram Positive Control Sample Capture Antibody User Manual
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 Âµl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation100 ml and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Orbital shaker Aluminum foil Saran Wrap Absorbent paper Microplate reader capable of measuring absorbance at 450nm Sigma Plot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline	Prepare all reagents, samples and standards as instructed. Add 100 Âµl detection antibody to each well. Incubate 1.5 h at R T or O/ N at 4Â° C. Add 100 Âµl standard or sample to each well. Incubate 2.5 h at R T. Add 100 Âµl prepared streptavidin solution. Incubate 45 min at R T. Add 100 Âµl T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 Âµl Stop Solution to each well. Read plate at 450 nm immediately.
Protein Name & Synonyms	Kininogen-1 (Alpha-2-thiol proteinase inhibitor) (Fitzgerald factor) (High molecular weight kininogen) (HMWK) (Williams-Fitzgerald-Flaujeac factor) [Cleaved into: Kininogen-1 heavy chain T-kinin (Ile-Ser-Bradykinin) Bradykinin (Kallidin I) Lysyl-bradykinin (Kallidin II) Kininogen-1 light chain Low molecular weight growth-promoting factor]
Specicifity	This EIA kit is designed to detect human, mouse, and rat Dynorphin
Recommended Dilution (serum & plasma)	Sample extraction

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

EIA-BRAK-1.pdf

EIA-BRAK-1-datasheet.pdf

EIA-BRAK-1-safety-datasheet.pdf