Comparison

Mouse Apoptosis Signaling Pathway Array C1

Item no. AAM-APOSIG-1-4
Manufacturer Raybiotech
Amount 4 Sample Kit
Quantity options 2 Sample Kit 4 Sample Kit 8 Sample Kit
Category
Type Array
Specific against Mouse (Murine, Mus musculus)
Host Hamster - Armenian
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Shipping condition Cool pack
Available
Manufacturer - Applications
Multiplexed Protein Detection
Detection of Relative Protein Expression
Detecting Patterns of Cytokine Expression
Biomarker/ Key Factor Screening
Identifying Key Factors
Confirming a Biological Process
Number of Targets Detected
17
Compatible Sample Types
Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates
Design Principle
Sandwich-based
Method of Detection
Chemiluminescence
Quantitative/Semi-Quantitative
Semi-Quantitative
Solid Support
Membrane
Gene Symbols
AKT1 | ATM | BAD | CASP3 | CASP7 | CDKN1B | CHEK1 | EIF2S1 | HSPB1 | MAP3K7|Â MAPK14 | MAPK8 | NFKBIA | RELA | SMAD2 | TP53
Kit Components
Mouse Apoptosis Signaling Pathway Antibody Array C1 Membranes
Blocking Buffer
Detection Antibody Cocktail1, 000
X H
R
P-
Anti-
Rabbit Ig
G Concentrate20
X Wash Buffer I Concentrate20
X Wash Buffer I
I Concentrate2
X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D8-
Well Incubation Tray w/ Lid
Protease Inhibitor Cocktail100x Phosphatase Inhibitor Cocktail I
Phosphatase Inhibitor Cocktail I
I
Plastic Sheets
Array Map Template
Manual
Other Materials Required
Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as U
V
P's Chemi
Doc-
It® or Epi
Chem I
I Benchtop Darkroom or G
E's Image
Quant„¢ L
A
S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with H
R
P-
Conjugated anti-
Ig
G
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Pathway
Akt SignalingHER/ErbB SignalingHIF-1 alpha SignalingIGF SignalingJAK/STAT SignalingMAPK SignalingmTOR SignalingNFkB Signalingp53 SignalingPI3K-AKT SignalingPKC SignalingTGF-beta Signaling

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 4 Sample Kit
Available: In stock
available

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