Item no. |
AAM-APOSIG-1-2 |
Manufacturer |
Raybiotech
|
Amount |
2 Sample Kit |
Quantity options |
2 Sample Kit
4 Sample Kit
8 Sample Kit
|
Category |
|
Type |
Array |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Hamster - Armenian |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Shipping condition |
Cool pack |
Available |
|
Manufacturer - Applications |
Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process |
Number of Targets Detected |
17 |
Compatible Sample Types |
Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates |
Design Principle |
Sandwich-based |
Method of Detection |
Chemiluminescence |
Quantitative/Semi-Quantitative |
Semi-Quantitative |
Solid Support |
Membrane |
Gene Symbols |
AKT1 | ATM | BAD | CASP3 | CASP7 | CDKN1B | CHEK1 | EIF2S1 | HSPB1 | MAP3K7|Â MAPK14 | MAPK8 | NFKBIA | RELA | SMAD2 | TP53 |
Kit Components |
Mouse Apoptosis Signaling Pathway Antibody Array C1 Membranes Blocking Buffer Detection Antibody Cocktail1, 000 X H R P- Anti- Rabbit Ig G Concentrate20 X Wash Buffer I Concentrate20 X Wash Buffer I I Concentrate2 X Cell Lysis Buffer Concentrate Detection Buffer C Detection Buffer D8- Well Incubation Tray w/ Lid Protease Inhibitor Cocktail100x Phosphatase Inhibitor Cocktail I Phosphatase Inhibitor Cocktail I I Plastic Sheets Array Map Template Manual |
Other Materials Required |
Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P's Chemi Doc- It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant„¢ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system. |
Protocol Outline |
Block membranes Incubate with Sample Incubate with Detection Antibody Cocktail Incubate with H R P- Conjugated anti- Ig G Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis |
Storage/Stability |
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Pathway |
Akt SignalingHER/ErbB SignalingHIF-1 alpha SignalingIGF SignalingJAK/STAT SignalingMAPK SignalingmTOR SignalingNFkB Signalingp53 SignalingPI3K-AKT SignalingPKC SignalingTGF-beta Signaling |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.