Item no. |
AAH-INSR-1-8 |
Manufacturer |
Raybiotech
|
Amount |
8 Sample Kit |
Quantity options |
2 Sample Kit
4 Sample Kit
8 Sample Kit
|
Category |
|
Type |
Array |
Specific against |
Human (Homo sapiens) |
Host |
Hamster - Armenian |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Shipping condition |
Cool pack |
Available |
|
Manufacturer - Applications |
Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process |
Compatible Sample Types |
Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates |
Design Principle |
Sandwich-based |
Method of Detection |
Chemiluminescence |
Quantitative/Semi-Quantitative |
Semi-Quantitative |
Solid Support |
Membrane |
Gene Symbols |
EIF4EFOXO3IGF1RINPP5DINSIRS1PTPN11SHC1STK11 |
Kit Components |
Human Insulin Receptor Phosphorylation Array C1 Membranes Blocking Buffer Detection Antibody Cocktail1, 000 X H R P- Anti- Rabbit Ig G Concentrate20 X Wash Buffer I Concentrate20 X Wash Buffer I I Concentrate2 X Cell Lysis Buffer Concentrate Detection Buffer C Detection Buffer D8- Well Incubation Tray w/ Lid Protease Inhibitor Cocktail100x Phosphatase Inhibitor Cocktail I Phosphatase Inhibitor Cocktail I I Plastic Sheets Array Map Template Manual |
Other Materials Required |
Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V Ps Chemi Doc- It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant™ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system. |
Protocol Outline |
Block membranes Incubate with Sample Incubate with Detection Antibody Cocktail Incubate with H R P- Conjugated anti- Ig G Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis |
Product Features |
Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex |
Storage/Stability |
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Pathway |
Akt SignalingIGF SignalingmTOR SignalingPI3K-AKT Signaling |
Typical Data |
Figure 1 - HepG2 or HeLa cells were grown to 80% confluency and then serum starved overnight. HepG2 Cells were treated with IGF for 10 minutes. HeLa Cells were treated with Calyculin A for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.