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Human Insulin Receptor Pathway Phosphorylation Array C1

Human Insulin Receptor Pathway Phosphorylation Array C1

Item no.	AAH-INSR-1-4
Manufacturer	Raybiotech
Amount	4 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Category	Oncology Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite By Cell Type Blastoma Carcinoma Endocrine Regulation of Lipid Metabolism Arrays Antibody Arrays
Type	Array
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Shipping condition	Cool pack
Available
Manufacturer - Applications	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	EIF4EFOXO3IGF1RINPP5DINSIRS1PTPN11SHC1STK11
Kit Components	Human Insulin Receptor Phosphorylation Array C1 Membranes Blocking Buffer Detection Antibody Cocktail1, 000 X H R P- Anti- Rabbit Ig G Concentrate20 X Wash Buffer I Concentrate20 X Wash Buffer I I Concentrate2 X Cell Lysis Buffer Concentrate Detection Buffer C Detection Buffer D8- Well Incubation Tray w/ Lid Protease Inhibitor Cocktail100x Phosphatase Inhibitor Cocktail I Phosphatase Inhibitor Cocktail I I Plastic Sheets Array Map Template Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V Ps Chemi Doc- ItÂ® or Epi Chem I I Benchtop Darkroom or G E's Image Quantâ„¢ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Detection Antibody Cocktail Incubate with H R P- Conjugated anti- Ig G Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Product Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Storage/Stability	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Pathway	Akt SignalingIGF SignalingmTOR SignalingPI3K-AKT Signaling
Typical Data	Figure 1 - HepG2 or HeLa cells were grown to 80% confluency and then serum starved overnight. HepG2 Cells were treated with IGF for 10 minutes. HeLa Cells were treated with Calyculin A for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

AAH-INSR-1-4.pdf

AAH-INSR-1-4-datasheet.pdf

AAH-INSR-1-4-safety-datasheet.pdf