Comparison

Rat Cytokine Array Q2

Item no. QAR-CYT-2-1
Manufacturer Raybiotech
Amount 8 Sample Kit
Quantity options 8 Sample Kit 22 Sample Kit 50 Sample Kit
Category
Type Array
Specific against Rat
Host Hamster - Armenian
Citations Driesen R., et al. Reversible and irreversible differentiation of cardiac fibroblasts. Cardiovascular Research (2014) 101, 411-422. DOI:10.1093/cvr/cvt338
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Similar products IFN-gamma, IL-10, IL-1 beta, IL-6, Leptin, TIMP-1, IL-6, MCP-1, ICAM-1, TNF alpha, L-selectin
Available
Short description
Quantibody Rat Cytokine Array 2 Kit. Detects 10 Rat Cytokines. Suitable for all liquid sample types.
Features
  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 uL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation
Number of Targets Detected
10
Target Names
ICAM-1 (CD54), IFN-gamma, IL-1 beta (IL-1 F2), IL-10, IL-6, Leptin, L-Selectin (CD62L), MCP-1 (CCL2), TIMP-1, TNF alpha
Targets Detected
CCL2, CD54, ICAM-1, IFN-gamma, IL-1 beta, IL-1 F2, IL-10, IL-6, L-Selectin, Leptin, MCP-1, TIMP-1, TNF alpha
Suggested Applications
Multiplexed Protein Detection, Biomarker Screening, Identifying Key Factors, Confirming Biological Process, Biomarker Validation, Validation of Antibody Array Results, Quantitative Protein Detection, Establishing Normal Range
Kit Components
  • Rat Cytokine Array Q2 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Sandwich-based
Result Output
Quantitative
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
Driesen R., et al. Reversible and irreversible differentiation of cardiac fibroblasts. Cardiovascular Research (2014) 101, 411-422. DOI:10.1093/cvr/cvt338

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 8 Sample Kit
Available: In stock
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