Comparison

Rat Cytokine Array C2

Item no. AAR-CYT-2-8
Manufacturer Raybiotech
Amount 8 Sample Kit
Quantity options 2 Sample Kit 4 Sample Kit 8 Sample Kit
Category
Type Array
Specific against Rat
Host Hamster - Armenian
Citations Wu, F., Zhang, R. et al. Preliminary Study on Pain Reduction of Monosodium Iodoacetate-Induced Knee Osteoarthritis in Rats by Carbon Dioxide Laser Moxibustion. Evidence-Based Complementary and Alternative Medicine 2014. Epub Ahead of Print. DOI: http://dx.doi.org/10.1155/2014/754304
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
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Available
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle
Sandwich-based
Detection Method
Chemiluminescence
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Kit Components
  • Rat Cytokine Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Number of Targets Detected
34
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Result Output
Semi-Quantitative
Short description
RayBio C-Series Rat Cytokine Antibody Array 2 Kit. Detects 34 Rat Cytokines. Suitable for all liquid sample types.
Solid Support
Membrane
Storage
For best results, store the entire kit frozen a -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected
Activin A, Agrin, B7-2, beta-NGF, CCL2, CCL20, CD54, CD62L, CD86, CINC-1, CINC-2, CINC-3, CNTF, CX3CL1, CXCL1, CXCL7, Fas Ligand, Fractalkine, GM-CSF, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 R6, IL-1 Rrp2, IL-10, IL-13, IL-2, IL-4, IL-6, L-Selectin, Leptin, LIX, MCP-1, MIP-3 alpha, MMP-8, PDGF-AA, Prolactin R, RAGE, TCK-1, TIMP-1, TNF alpha, TNFSF6, VEGF-A

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 8 Sample Kit
Available: In stock
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