Item no. |
QAP-CYT-1-2 |
Manufacturer |
Raybiotech
|
Amount |
22 Sample Kit |
Quantity options |
8 Sample Kit
22 Sample Kit
50 Sample Kit
|
Category |
|
Type |
Array |
Specific against |
Pig |
Host |
Hamster - Armenian |
Citations |
1. Beltran-Beck B., de la Fuente J., Garrido J., Aranaz A., Sevilla I., et al. Oral Vaccination with Heat Inactivated Mycobacterium bovis Activates the Complement System to Protect against Tuberculosis. PLOS One, 2014 May 19, DOI: 10.1371/journal.pone.0098048 |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Similar products |
IFN-gamma, IL-10, IL-1 beta, IL-6, IL-4, IL-12, IL-8, IL-6, GM-CSF, TGF beta 1, TNF alpha |
Available |
|
Number of Targets Detected |
10 |
Species Detected |
Porcine (pig) |
Compatible Sample Types |
Cell Culture Supernatants Cell Lysates Other Body Fluids Plasma Serum Tissue Lysates |
Design Principle |
Sandwich-based |
Method of Detection |
Fluorescence Laser Scanner |
Quantitative/Semi-Quantitative |
Quantitative |
Solid Support |
Glass Slide |
Gene Symbols |
CSF2CXCL8IFNGIL10IL12AIL1BIL4IL6TGFB1TNF |
Application Information |
Multiplexed Protein Detection Biomarker Screening Identifying Key Factors Confirming Biological Process Biomarker Validation Validation of Antibody Array Results Quantitative Protein Detection Establishing Normal Range |
Kit Components |
Porcine Cytokine Array Q1 Slide(s) Blocking Buffer Wash Buffer 1 Wash Buffer 2 Lyophilized Standard Mix Biotinylated Detection Antibody Cocktail Streptavidin- Conjugated Fluor Slide Washer/ Dryer Adhesive Plastic Strips Manual |
Other Materials Required |
Distilled or deionized water Small plastic boxes or containers Pipettors, pipette tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Gene microarray scanner or similar laser fluorescence scanner |
Protocol Outline |
Dry the glass slide Prepare Standards Block array surface Incubate with Samples and Standards Incubate with Biotinylated Detection Antibody Cocktail Incubate with Streptavidin- Conjugated Fluor Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis |
Storage/Stability |
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Pathway |
JAK/STAT SignalingMAPK SignalingNFkB Signaling |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.