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Mouse Cytokine Array C5

Mouse Cytokine Array C5

Item no.	AAM-CYT-5-8
Manufacturer	Raybiotech
Amount	8 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience Aging & Neurological Disorders Neural Adhesion Molecules By Cell Type Carcinoma Hematology Leukemia Lymphoma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Cardiovascular Research Heart Failure Myocardial Infarction Arrays By Organ Bladder Cancer Renal Cancer Antibody Arrays
Type	Array
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Citations	Verhoeven, D., Pichichero, M., et al. Divergent Mucosal and Systemic Responses in Children in Response to Acute Otitis Media. Clinical & Experimental Immunology 2014 May Epub Ahead of Print DOI: 10.1111/cei.12389
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	EGF, HAI-1, HGF, RAGE, Epiregulin, Prolactin, TACI, IL-21, TWEAK, CD27, E-Cadherin, CD40 Ligand, Endoglin, Granzyme B, IL-11, Amphiregulin, DKK-1, Epigen, IL-17E, IL-20, 4-1BB, IL-17B, CTLA-4, CD36, Galectin-1, Neprilysin, Cardiotrophin-1, Chordin, MAdCAM-1, Decorin, Pentraxin 3, 6Ckine, TREM-1, ALK-1, MFG-E8, JAM-A, TWEAK R, IL-6 R, IL-28, IL-2 R alpha, GITR Ligand, CD27 Ligand, ACE /CD143, Growth arrest specific 1, Growth arrest specific 6, IL-1 R4/ST2L, L-17F, IL-1 ra/IL-1F3
Shipping condition	Cool pack
Available
Number of Targets Detected	48
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Solid Support	Membrane
Kit Components	Mouse Cytokine Antibody Array C5 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated HRP Detection Buffer C Detection Buffer D Lysis Buffer 8-Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Detection Method	Chemiluminescence
Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Result Output	Semi-Quantitative
Short description	RayBio C-Series Mouse Cytokine Antibody Array 5 Kit. Detects 48 Mouse Cytokines. Suitable for all liquid sample types.
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected	4-1BB, 6Ckine, ACE, ALK-1, Amphiregulin, Cardiotrophin-1, CCL21, CD105, CD152, CD27, CD27 Ligand, CD36, CD40 Ligand, Chordin, CT-1, CTLA-4, Decorin, DKK-1, E-Cadherin, EGF, Endoglin, Epigen, Epiregulin, Galectin-1, Gas 1, Gas 6, GITR Ligand, Granzyme B, HAI-1, HGF, IFN-lambda, IL-1 F3, IL-1 R4, IL-1 Ra, IL-11, IL-17B, IL-17C, IL-17E, IL-17F, IL-2 R alpha, IL-20, IL-21, IL-28, IL-6 R, JAM-A, MAdCAM-1, MFG-E8, Neprilysin, Pentraxin-3, Prolactin, RAGE, SR-B3, ST2, TACI, TNFRSF13B, TNFRSF7, TNFRSF9, TNFSF12, TNFSF18, TNFSF5, TNFSF7, TREM-1, TSG-14, TWEAK, TWEAK R

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.