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Mouse Cytokine Array C2

Mouse Cytokine Array C2

Item no.	AAM-CYT-2-2
Manufacturer	Raybiotech
Amount	2 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Neural Signaling By Cell Type Carcinoma Glioma Hematology Leukemia Lymphoma Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Adenocarcinoma Endocrine Regulation of Lipid Metabolism Arrays By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Testicular & Prostate Cancer Antibody Arrays
Type	Array
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Citations	1. Zhu D., Can C., Purwanti Y., Du S., Lam D., Wu C., et al. Induced Pluripotent Stem Cell-derived Neural Stem Cells Transduced with Baculovirus Encoding CD40 Ligand for Immunogene Therapy in Mouse Models of Breast Cancer. Human Gene Therapy Online Ahead of Editing: April 28th, 2014. doi:10.1089/hum.2013.160. [Epub ahead of print] 2. Honko AN, Mizel SB. Mucosal Administration of Flagellin Induces Innate Immunity in the Mouse Lung. Infect Immun. 2004,72(11):6676-6679. 3. Rich J, Lee J. The Pathogenesis of Staphylococcus aureus Infection in the Diabetic NOD Mouse. Diabetes. 2005,54:2904–2910. 4. Wu, Q et al. Monitoring the response of orthotopic bladder tumors to granulocyte macrophage colony-stimulating factor therapy using the prostate-specific antigen gene as a reporter. Monitoring the response of orthotopic bladder tumors to granulocyte macrophage colony-stimulating factor therapy using the prostate-specific antigen gene as a reporter. Monitoring the response of orthotopic bladder tumors to granulocyte macrophage colony-stimulating factor therapy using the prostate-specific antigen gene as a reporter. Clin. Cancer Res. Volume 10, Issue 20, Pages 6977-84, Oct 2004 5. Fan, H et al. Cytokine dysregulation induced by apoptotic cells is a shared characteristic of macrophages from nonobese diabetic and systemic lupus erythematosus-prone mice. J. Immunol. Volume 172, Issue 8, Pages 4834-43, Apr 2004 6. Michel, M., et al. Identification of an IL-17–producing NK1.1neg iNKT cell population involved in airway neutrophilia. Journal of experimental Medicine. 2007, vol. 204 no. 5 995-1001. doi: 10.1084/jem.20061551 7. Jung Y., Kim M., Soh H., Lee S., et al. TC1(C8orf4) Regulates Hematopoietic Stem/Progenitor Cells and Hematopoiesis. PLOS One. Published: June 17, 2014 DOI: 10.1371/journal.pone.0100311 8. Herrmann, A., Priceman, S., et al. CTLA4 aptamer delivers STAT3 siRNA to tumor-associated and malignant T cells. The Journal of Clinical Investigation 2014. Epub Ahead of Print DOI: 10.1172/JCI73174 9. Ehninger D. Tsc2 Haploinsufficiency Has Limited Effects on Fetal Brain Cytokine Levels during Gestational Immune Activation. Autism Research and Treatment. Volume 2014, Article ID 761279, 7 pages http://dx.doi.org/10.1155/2014/761279 10. Shan Y., er al. Suppression of mucin 2 promotes interleukin-6 secretion and tumor growth in an orthotopic immune-competent colon cancer animal model. ONCOLOGY REPORTS 32: 2335-2342, 2014, DOI: 10.3892/or.2014.3544
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
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Shipping condition	Cool pack
Available
Manufacturer - Applications	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process
Number of Targets Detected	32
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	CCL11 \| CCL12 \| CCL17 \| CCL19 \| CCL2 \| CCL20 \| CCL21 \| CCL27 \| CCL3 \| CCL5\|Â CSF2 \| CSF3 \| CXCL1 \| CXCL2 \| IFNG \| IL10 \| IL12A \| IL13 \| IL2 \| IL3 \| IL4 \| IL5 \| IL6 \| IL9 \| KITLG \| LEP \| THPO \| TIMP1 \| TNF \| TNFRSF1A \| TPO \| VEGFA
Kit Components	Mouse Cytokine Antibody Array C2 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin- Conjugated H R P Detection Buffer C Detection Buffer D Lysis Buffer8- Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P??s Chemi Doc- ItÂ® or Epi Chem I I Benchtop Darkroom or G E's Image Quantâ„¢ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.