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Mouse Cytokine Array C1

Mouse Cytokine Array C1

Item no.	AAM-CYT-1-8
Manufacturer	Raybiotech
Amount	8 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit 2 Sample Kit 4 Sample Kit 8 Sample Kit
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis By Cell Type Carcinoma Hematology Leukemia Lymphoma Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Adenocarcinoma Arrays By Organ Lung Cancer Bladder Cancer Antibody Arrays
Type	Array
Specific against	Mouse
Host	Hamster - Armenian
Citations	1. Wang M, Xiao GG, Li N, Xie Y, Loo JA, Nel AE. Use of a fluorescent phosphoprotein dye to characterize oxidative stress-induced signaling pathway components in macrophage and epithelial cultures exposed to diesel exhaust particle chemicals. Electrophoresis. 2005,26:2092–2108.<br />2. Carnevalli L., Scognamiglio R., Cabezas-Wallschied N., Rahmig S., Laurenti E. Improved HSC reconstitution and protection from inflammatory stress and chemotherapy in mice lacking granzyme B. JEM vol. 211 no. 5 769-779 <br />3. Park J., Li H., Zhang M., Lu Y., Hong B., et al. Murine Th9 cells promote the survival of myeloid dendritic cells in cancer immunotherapy. Cancer Immunology and Immunotherapy, 2014 Epub Ahead of Print. DOI 10.1007/s00262-014-1557-4 <br />4. Resa-Infante P., Thieme R., Ernst T., Arck P., Ittrich H., Reimer R., Gabriel G. Importin-alpha7 is Required for Enhanced Influenza A Virus Replication in the Alveolar Epithelium and Severe Lung Damage in Mice. Journal of Virology Volume 88, Issue 11.<br />5. Le Quement, C., Guenon, I., Gillon, J.-Y., Valença, S., Cayron-Elizondo, V., Lagente, V. and Boichot, E. (2008), The selective MMP-12 inhibitor, AS111793 reduces airway inflammation in mice exposed to cigarette smoke. British Journal of Pharmacology, 154: 1206–1215. doi: 10.1038/bjp.2008.180<br />6. Kimura H, Tzou S-C, Rocchi R, Kimura M, et al. Interleukin (IL)-12-Driven Primary Hypothyroidism: the Contrasting Roles of Two Th1 Cytokines (IL-12 and Interferon-gamma). Endocrinol. 2005,146(8):3642–3651.<br />7. Kim B., Kim H. Chrysanthemum zawadskii extract activates peroxisome proliferator-activated receptor-alpha. Korean J. Chem. Eng., 31(4), 1-8 (2014). DOI: 10.1007/s11814-014-0109-0 and has an anti-inflammatory activity : Potential interest for the skin barrier function<br />8. Denis Gallagher, Andreea A. Norman, Cameron L. Woodard, et al. "Transient Maternal IL-6 Mediates Long-Lasting Changes in Neural Stem Cell Pools by Deregulating an Endogenous Self-Renewal Pathway." Cell Stem Cell 13, 564–576, November 7, 2013. http://dx.doi.org/10.1016/j.stem.2013.10.002
ECLASS 5.1	32161000
ECLASS 6.1	32161000
ECLASS 8.0	32161000
ECLASS 9.0	32161000
ECLASS 10.0.1	32161000
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	IFN-gamma, IL-10, IL-6, VEGF, SCF, IL-13, IL-4, IL-6, G-CSF, IL-17, IL-2, RANTES, GM-CSF, IL-5, IL-9, MCP-1, IL-3, IL-12 p70, MCP-5, Thrombopoietin, TNF alpha, sTNFRI, IL-12 p40/p70
Available
Number of Targets Detected	22
Species Detected	Mouse
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	CCL12 \| CCL2 \| CCL5 \| CSF2 \| CSF3 \| IFNG \| IL10 \| IL12A \| IL13 \| IL17A\| IL2 \| IL3 \| IL4 \| IL5 \| IL6 \| IL9 \| KITLG \| THPO \| TNF \| TNFRSF1A \| VEGFA
Application Information	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process
Kit Components	Mouse Cytokine Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin- Conjugated H R P Detection Buffer C Detection Buffer D Lysis Buffer8- Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P's Chemi Doc- It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant™ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Product Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.