Human Glycosylation Array 507

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Fluorescence Laser Scanner

Glass Slide

Human Glycosylation Array 507 Glass Slide/s
Dilution Buffer20
X Wash Buffer I20
X Wash Buffer I
I
Cy3 equivalent dye-conjugated Streptavidin
Biotin-labeled Lectin Mixture
Adhesive device sealer
Manual

Dry the glass slide
Incubate samples
Incubate with biotin lectin mixture
Incubate with Cy3 Equivalent Dye-
Streptavidin
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Recent technological advances by RayBiotech have enabled the largest commercially available glycosylation antibody array to date. With the Human Glycosylation Array 507, researchers can now efficiently obtain glycosylation profiles in their samples. The glycosylation profiles of 507 human proteins can be simultaneously detected, including, but not limited to, cytokines, chemokines, adipokines, growth factors, angiogenic factors, proteases, soluble receptors, soluble adhesion molecules in cell culture supernatants, serum, plasma and other samples types.Capture antibodies are printed onto glass slides and the glycans on these capture antibodies are removed. The glass slide arrays come pre-blocked and are ready to be incubated with samples. After incubation with samples and washing to remove unbound proteins, five unique biotin-labeled lectins are incubated with the array to allow binding to the glycans from captured target proteins. Streptavidin-conjugated fluorescent dye (Cy3 equivalent) is then added to the array. Finally, the glass slide is dried and laser fluorescence scanning is used to visualize the signals. These signals are then compared to the array map to identify glycosylated proteins present in the samples.

Lectin Name
Sugar specificity