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Human RTK Phosphorylation Array C1

Human RTK Phosphorylation Array C1

Item no.	AAH-PRTK-1-4
Manufacturer	Raybiotech
Amount	4 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Category	Oncology Neuroscience Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Adenocarcinoma Myelofibrosis Endocrine Regulation of Lipid Metabolism Arrays Astrocytoma By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Heart & Blood Cancer Renal Cancer Antibody Arrays
Type	Array
Specific against	Human
Host	Hamster - Armenian
Citations	Acquired resistance to sunitinib in human renal cell carcinoma cells is mediated by constitutive activation of signal transduction pathways associated with tumour cell proliferation
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	EphA2, EphB2, FGFR2, RYK, TYRO10, RET, SYK, EGFR, FGFR1, VEGFR2, ZAP70, JAK1, LCK, MUSK, FAK, ALK, Axl, Csk, Fgr, FRK, Fyn, Hck, Itk, Lyn, MATK, ROR1, Tec, Tyk2, ROR2, JAK2, JAK3, LTK, TNK1, ABL1, SCFR, VEGFR3, ACK1, Tie-1, ROS, BMX, FER, SRMS, TXK, Blk, ErbB3, ErbB2, TRKB, EphA7, EphA8, EphB1, EphB6, IGF-1 R, Tie-2, Btk, PYK2, Dtk, EphB4, ErbB4, EphA6, EphB3, M-CSF R, EphA5, EphA4, EphA3, EphA1, Insulin R, NGF R, HGF R, FGFR2 (alpha isoform), PDGF R-alpha, PDGF R-beta
Available
Number of Targets Detected	71
Species Detected	Human
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	ABL1 \| AXL \| BLK \| BMX \| BTK \| CSF1R \| CSK \| DDR2 \| EGFR \| EPHA1\| EPHA2 \| EPHA3 \| EPHA4 \| EPHA5 \| EPHA6 \| EPHA7 \| EPHA8 \| EPHB1 \| EPHB2 \| EPHB3 \| EPHB4 \| EPHB6 \| ERBB2 \| ERBB3 \| ERBB4 \| FER \| FGFR1 \| FGFR2 \| FGR \| FLT4 \| FRK \| FYN \| HCK \| IGF1R \| INSR \| ITK \| JAK1 \| JAK2 \| JAK3 \| KDR \| KIT \| LCK \| LTK \| LYN \| MATK \| MET \| MUSK \| NGFR \| NTRK2 \| PDGFRA \| PDGFRB \| PTK2 \| PTK2B \| RET \| ROR1 \| ROR2 \| ROS1 \| RYK \| SLPI \| SRMS \| SYK \| TEC \| TEK \| TIE1 \| TNK1 \| TNK2 \| TXK \| TYK2 \| TYRO3 \| ZAP70
Application Information	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process
Kit Components	Human R T K Phosphorylation Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Anti- Phospho Tyrosine Detection Antibody Streptavidin- Conjugated H R P Detection Buffer C Detection Buffer D Lysis Buffer (1 x 5 ml) Protease Inhibitor Cocktail Phosphatase Inhibitor Cocktail I I8- Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P??s Chemi Doc- It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant™ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Product Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Pathway	PI3K-AKT Signaling

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

AAH-PRTK-1-4.pdf

AAH-PRTK-1-4-datasheet.pdf

AAH-PRTK-1-4-safety-datasheet.pdf