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Human RTK Phosphorylation Array C1

Human RTK Phosphorylation Array C1

Manufacturer	Raybiotech
Category	Oncology Neuroscience Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Adenocarcinoma Myelofibrosis Endocrine Regulation of Lipid Metabolism Arrays Astrocytoma By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Heart & Blood Cancer Renal Cancer Antibody Arrays
Type	Assay
Specific against	Human
Amount	2 Sample Kit
Item no.	AAH-PRTK-1-2
Targets	INS ;; KDR ;; NGF ;; PHB ;; TEK ;;CD117;;CD220;;CD331;;CD332;;EPHA3;;ERBB2;;ERBB3;;PTK2B;;VEGFA
eClass 6.1	32161000
eClass 9.0	32161000
Available
Short description	RayBio C-Series Human Receptor Tyrosine Kinase Phosphorylation Antibody Array 1 Kit. Detects 71 Human RTKs. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Secondary categories	Products, Functional Assays, Phosphorylation Assays, Phosphorylation Arrays
Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected	71
Target Names	ABL1, ACK, ALK-1, Axl, Blk, BMX, Btk, Csk, Dtk, EGFR, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ErbB2, ErbB3, ErbB4, FAK, FER, FGFR1, FGFR2, FGFR2, Fgr, FRK, Fyn, Hck, HGFR, IGF-1 R, Insulin R (CD220), Itk, JAK1, JAK2, JAK3, LCK, LTK, Lyn, MATK, M-CSF R, MUSK, NGFR (TNFRSF16), PDGFRA, PDGFRB, PYK2, RET, ROR1, ROR2, ROS, RYK, SCF R (CD117/c-kit), SRMS, SYK, Tec, Tie-1, Tie-2, TNK1, TRKB, TXK, Tyk2, TYRO10 (DDR2/TKT), VEGFR2, VEGFR3
Targets Detected	ABL1, ACK1, ALK-1, AXL, Blk, BMX, Btk, c-kit, CD117, CD220, CSK, Dtk, EGFR, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ErbB1, ErbB2, ErbB3, ErbB4, FAK, FER, FGFR1, FGFR2, FGFR2 alpha, Fgr, FRK, Fyn, Hck, HGFR, IGF-1 R, Insulin R, ITK, JAK1, JAK2, JAK3, LCK, LTK, Lyn, M-CSF R, MATK, MUSK, NGFR, PDGF R alpha, PDGF R beta, PYK2, RET, ROR1, ROR2, ROS, RYK, SCF R, SRMS, SYK, Tec, Tie-1, Tie-2, TNFRSF16, TNK1, TRKB, TXK, Tyk2, TYRO10, VEGFR2, VEGFR3, ZAP70
Suggested Applications	Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components	Human RTK Phosphorylation Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Anti-Phospho Tyrosine Detection Antibody Streptavidin-Conjugated HRP Detection Buffer C Detection Buffer D Lysis Buffer (1 x 5 ml) Protease Inhibitor Cocktail Phosphatase Inhibitor Cocktail II 8-Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Storage	For best results, store the entire kit frozen a -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support	Membrane
Design Principle	Sandwich-based
Result Output	Semi-Quantitative
Detection Method	Chemiluminescence
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation	Acquired resistance to sunitinib in human renal cell carcinoma cells is mediated by constitutive activation of signal transduction pathways associated with tumour cell proliferation

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.