Item no. |
AAH-PRTK-1-2 |
Manufacturer |
Raybiotech
|
Amount |
2 Sample Kit |
Quantity options |
2 Sample Kit
4 Sample Kit
8 Sample Kit
|
Category |
|
Type |
Array |
Specific against |
Human |
Host |
Hamster - Armenian |
Citations |
Acquired resistance to sunitinib in human renal cell carcinoma cells is mediated by constitutive activation of signal transduction pathways associated with tumour cell proliferation |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Similar products |
EphA2, EphB2, FGFR2, RYK, TYRO10, RET, SYK, EGFR, FGFR1, VEGFR2, ZAP70, JAK1, LCK, MUSK, FAK, ALK, Axl, Csk, Fgr, FRK, Fyn, Hck, Itk, Lyn, MATK, ROR1, Tec, Tyk2, ROR2, JAK2, JAK3, LTK, TNK1, ABL1, SCFR, VEGFR3, ACK1, Tie-1, ROS, BMX, FER, SRMS, TXK, Blk, ErbB3, ErbB2, TRKB, EphA7, EphA8, EphB1, EphB6, IGF-1 R, Tie-2, Btk, PYK2, Dtk, EphB4, ErbB4, EphA6, EphB3, M-CSF R, EphA5, EphA4, EphA3, EphA1, Insulin R, NGF R, HGF R, FGFR2 (alpha isoform), PDGF R-alpha, PDGF R-beta |
Available |
|
Short description |
RayBio C-Series Human Receptor Tyrosine Kinase Phosphorylation Antibody Array 1 Kit. Detects 71 Human RTKs. Suitable for all liquid sample types but intended for use with cell and tissue lysates. |
Secondary categories |
Products, Functional Assays, Phosphorylation Assays, Phosphorylation Arrays |
Features |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
|
Number of Targets Detected |
71 |
Target Names |
ABL1, ACK, ALK-1, Axl, Blk, BMX, Btk, Csk, Dtk, EGFR, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ErbB2, ErbB3, ErbB4, FAK, FER, FGFR1, FGFR2, FGFR2, Fgr, FRK, Fyn, Hck, HGFR, IGF-1 R, Insulin R (CD220), Itk, JAK1, JAK2, JAK3, LCK, LTK, Lyn, MATK, M-CSF R, MUSK, NGFR (TNFRSF16), PDGFRA, PDGFRB, PYK2, RET, ROR1, ROR2, ROS, RYK, SCF R (CD117/c-kit), SRMS, SYK, Tec, Tie-1, Tie-2, TNK1, TRKB, TXK, Tyk2, TYRO10 (DDR2/TKT), VEGFR2, VEGFR3 |
Targets Detected |
ABL1, ACK1, ALK-1, AXL, Blk, BMX, Btk, c-kit, CD117, CD220, CSK, Dtk, EGFR, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ErbB1, ErbB2, ErbB3, ErbB4, FAK, FER, FGFR1, FGFR2, FGFR2 alpha, Fgr, FRK, Fyn, Hck, HGFR, IGF-1 R, Insulin R, ITK, JAK1, JAK2, JAK3, LCK, LTK, Lyn, M-CSF R, MATK, MUSK, NGFR, PDGF R alpha, PDGF R beta, PYK2, RET, ROR1, ROR2, ROS, RYK, SCF R, SRMS, SYK, Tec, Tie-1, Tie-2, TNFRSF16, TNK1, TRKB, TXK, Tyk2, TYRO10, VEGFR2, VEGFR3, ZAP70 |
Suggested Applications |
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process |
Kit Components |
- Human RTK Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Anti-Phospho Tyrosine Detection Antibody
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer (1 x 5 ml)
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail II
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
|
Other Materials Required |
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
|
Protocol Outline |
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
|
Storage |
For best results, store the entire kit frozen a -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20C and all other reagents undiluted at 4C for no more than 3 months. |
Solid Support |
Membrane |
Design Principle |
Sandwich-based |
Result Output |
Semi-Quantitative |
Detection Method |
Chemiluminescence |
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Citation |
Acquired resistance to sunitinib in human renal cell carcinoma cells is mediated by constitutive activation of signal transduction pathways associated with tumour cell proliferation |